| 亚硝酸盐降解菌N419的培养基优化 |
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| 引用本文： | 许丽娟,陈薇,吴迎奔,王震,汪彬,贺月林.亚硝酸盐降解菌N419的培养基优化[J].湖南农业科学,2016(9). |
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| 作者姓名： | 许丽娟 陈薇 吴迎奔 王震 汪彬 贺月林 |
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| 作者单位： | 湖南省微生物研究院,湖南 长沙,410009 |
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| 基金项目： | 湖南省科技计划(2015CK3008) |
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| 摘 要： | 为了规模化生产和实际应用亚硝酸盐降解菌N-419,对其发酵培养基进行了优化,选出由单因素试验确定的营养物质(玉米淀粉、工业蛋白胨)以及Ca Cl2和Mn SO4,进行4因素3水平的正交试验。结果表明:N-419菌株的最佳培养基配方为玉米淀粉20 g/L、工业蛋白胨10 g/L、Ca Cl20.3 g/L、Mn SO40.2 g/L、K2HPO4 0.12 g/L、KH2PO4 0.25 g/L,其芽孢数可达1.44×1010 CFU/m L。
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| 关 键 词： | 亚硝酸盐降解菌 枯草芽孢杆菌 培养基优化 |
Optimization of Culture Media for A Bacillus Subtilis of Degrading Nitrite |
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| Abstract: | In order to use nitrite degrading bacteria N-419 in scale production, ed its culture medium was optimized. According to the results of single factor orthogonal test for culture optimization, the orthogonal experiment with 4 factors (corn powder, industrial peptone, CaCl2, MnSO4) and 3 levels was carried out. The results showed that optimal medium was as follows: corn powder 20 g/L, industrial peptone 10 g/L, CaCl2 0.3 g/L, MnSO4 0.2 g/L, K2HPO4 0.12 g/L, KH2PO4 0.25 g/L, respectively. Under the conditions, 1.44×1010 CFU bacteria per milliliter were obtained. |
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| Keywords: | nitrite Bacillus subtilis medium optimization |
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