| 甘薯薯块DNA提取方法研究 |
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| 引用本文： | 张道微,张超凡,董芳,黄艳岚,张亚,周虹.甘薯薯块DNA提取方法研究[J].湖南农业科学,2016(4):5-7. |
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| 作者姓名： | 张道微 张超凡 董芳 黄艳岚 张亚 周虹 |
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| 作者单位： | 1. 湖南省作物研究所,湖南长沙,410125;2. 中南大学研究生院隆平分院,湖南长沙,410125 |
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| 基金项目： | 国家甘薯产业技术体系(CARS-11-C-16) |
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| 摘 要： | 甘薯薯块富含淀粉、酚类等物质,不利于DNA提取。为了研究甘薯薯块DNA提取的最佳方法,采用经典CTAB法、改良CTAB法一、改良CTAB法二和SDS提取法,对甘薯薯块总DNA提取效果进行比较,以试剂盒法提取结果作对照。结果表明:采用经典CTAB提取法和SDS提取法获得的薯块DNA含有较多杂质,且降解严重,样品质量低;CTAB改良法一能降低DNA样品中多糖等物质的含量,但DNA存在一定程度降解,且耗时较长;CTAB改良法二提取效果最佳,杂质含量低且DNA降解少,与试剂盒提取效果最接近,是一种较为理想的甘薯薯块DNA提取方法。
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| 关 键 词： | DNA提取 CTAB提取法 SDS提取法 |
Study on Extraction Method of DNA from Sweet Potato Tuber |
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| Abstract: | Potato tuber is rich in starch, phenol and other substances, which is not conducive to DNA extraction. In order to study the best extraction method of sweet potato tuber DNA, using the classical CTAB method, improved CTAB method-1, improved CTAB method-2 and SDS extraction method, to compare the sweet potato tuber total DNA extraction effect, use the extraction result of kit method as control. The results show that the classic CTAB extraction method and SDS extraction method contains more impurities, serious degradation and the quality of sample is low; the improved CTAB method-1 can reduce the content of polysaccharide and other substances in DNA samples, but DNA has a certain degree of degradation, and it consumes too much time; the improved CTAB method-2 has best extraction effect, low content of impurities and degradation of DNA, the closest extraction effect to kit method, is an ideal method for extracting DNA from sweet potato tuber. |
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| Keywords: | DNA extraction CTAB extraction method SDS extraction method |
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