| 玉米大斑病菌亲环素基因的克隆及表达规律分析 |
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| 引用本文： | 赵玉兰,李盼,魏宁,郝志敏,曹志艳,李志勇,董金皋.玉米大斑病菌亲环素基因的克隆及表达规律分析[J].玉米科学,2019,27(2):29-35. |
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| 作者姓名： | 赵玉兰 李盼 魏宁 郝志敏 曹志艳 李志勇 董金皋 |
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| 作者单位： | 河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室;河北省农林科学院谷子研究所 |
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| 基金项目： | 国家自然科学基金项目（31601598）、国家现代农业（玉米）产业技术体系（CARS-02-12）、河北省自然科学基金项目（C2016204160）、河北省高等学校青年拔尖人才计划项目（BJ2014349Y） |
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| 摘 要： | 利用简并引物PCR结合RACE技术获得S. turcica中亲环素基因的全长,并通过Real-time PCR技术检测该基因在病菌侵染结构发育过程中的表达模式。结果表明,玉米大斑病菌亲环素基因开放阅读框全长1 125 bp,3′UTR 154 bp,5′UTR 93 bp,编码374个氨基酸,将此基因命名为CyPs1,并将其cDNA序列提交GenBank,获得登录号EU679371.1,Protein ID为ACD62431.1。系统发育树分析显示,CyPs1与玉米小斑病菌(Bipolaris maydis)、蓝莓枯枝病菌(Neofusicoccum parvum)等物种的亲环素同源性可达到90%以上。该基因在病菌分生孢子萌发、附着胞形成及侵染阶段均有表达,至附着胞形成和侵染菌丝形成阶段,转录水平分别升至分生孢子时期的2倍和3倍。
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| 关 键 词： | 玉米 玉米大斑病菌 亲环素 Ca2+信号途径 附着胞 |
Cloning and Expression Analysis of Setosphaeria turcica Cyclophilin Gene |
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| ZHAO Yu-lan,LI Pan,WEI Ning,HAO Zhi-min,CAO Zhi-yan,LI Zhi-yong and DONG Jin-gao.Cloning and Expression Analysis of Setosphaeria turcica Cyclophilin Gene[J].Journal of Maize Sciences,2019,27(2):29-35. |
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| Authors: | ZHAO Yu-lan LI Pan WEI Ning HAO Zhi-min CAO Zhi-yan LI Zhi-yong and DONG Jin-gao |
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| Institution: | Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000,Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000,Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000,Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000,Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000,Millet Institute of Agricultural Academy of Hebei Province, Shijiazhuang 050031, China and Key Laboratory of Hebei Province for Plant Physiological and Molecular Pathology, College of Life Sciences, Agricultural University of Hebei, Baoding 071000 |
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| Abstract: | |
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| Keywords: | Maize Setosphaeria turcica Cyclophilin Ca2+ signaling Aappressorium |
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