| 8株布氏杆菌BCSP31基因的序列分析 |
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| 引用本文： | 丁家波,毛开荣,陈小云,程君生,戴志红,蒋玉文.8株布氏杆菌BCSP31基因的序列分析[J].中国兽药杂志,2006,40(3):13-16. |
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| 作者姓名： | 丁家波 毛开荣 陈小云 程君生 戴志红 蒋玉文 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 摘 要： | 参照GenBank中布氏杆菌的全基因组序列，设计一对特异性引物，通过PCR方法，扩增了8株来自3个布氏杆菌种的布氏杆菌表面蛋白31（BCSP31）全基因。PCR产物经克隆和序列分析后发现，该基因非常保守，8株不同菌株间的同源性高于99．3％；进化关系分析显示，5株中国来源的菌株（S2，M5，M111，M28，A387）显示了最近的同源关系，其中2个弱毒菌株S2和M5的BCSP31基因序列100％同源。3株外国来源的菌株（S19，2308，Rev．1）中，S19和2308同源性为100％。Rev．1与其他7株布氏杆菌BCSP31基因均有较大的差异。序列分析结果表明，BCSP31基因序列差异与布氏杆菌的种属和毒力无相关性。
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| 关 键 词： | 布氏杆菌 BCSP31基因 序列分析 |
| 文章编号： | 1002-1280（2006）03-0013-04 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-21 |
Sequence Analysis on BCSP31 Genes of 8 Different Brucella Species |
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| DING Jia-bo,MAO Kai-rong,CHEN Xiao-yun,CHENG Jun-sheng,DAI Zhi-hong,JIANG Yu-wen.Sequence Analysis on BCSP31 Genes of 8 Different Brucella Species[J].Chinese Journal of Veterinary Drug,2006,40(3):13-16. |
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| Authors: | DING Jia-bo MAO Kai-rong CHEN Xiao-yun CHENG Jun-sheng DAI Zhi-hong JIANG Yu-wen |
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| Institution: | China Institute of Veterinary Drug Control, Beijing 100081 ; China |
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| Abstract: | A pair of primers was synthesized for amplification of brucella cell surface protein 31 ku(BCSP31) gene according to the complete genomic sequence of Brucella abortus published in GenBank.BCSP31 genes were amplified from 8 Brucella strains belonging into 3 different species by polymerase chain reaction(PCR). The PCR products were cloned in pMD18-T vector for sequencing.Sequence analysis showed that BCSP31 genes were conservative,and the identical percent of these genes was more than 99.3% between these 8 strains.Evolution analysis(revealed) that 5 Chinese strains(S2,M5,M111,M28,A387) had the nearest relationship,among them 2(attenuated) strains,S2 and M5,had the same sequences in BCSP31.Among the 3 foreign strains(S19,2308,Rev.1),there was only little difference between S19 and 2308 in BCSP31 sequence.Rev.1 was significantly(different not) only from the 5 Chinese strains,but also from S19 and 2308.No relationships were found between the sequence divergence of BCSP31 gene and the species and virulence of Brucellas. |
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| Keywords: | Brucella BCSP31 gene sequence analysis |
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