Positive Effect of Manganese (III) Meso-Tetrakis (4-Benzoic Acid) Porphyrin on Stallion Spermatozoa During Storage in Cool Condition |
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| Institution: | 1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Animal Production, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt;1. Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland;2. Department and Clinic of Animal Reproduction, Sub-Department of Andrology and Biotechnology of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland;1. Université Paris-Est, Ecole Nationale Vétérinaire d''Alfort, CIRALE-Hippolia, Goustranville, France;2. INRA, USC BPLC 957, Maisons-Alfort, France;3. Université Paris-Est, Ecole Nationale Vétérinaire d''Alfort, Maisons-Alfort, France;4. OTR3, Paris, France;1. Centro de Investigación en Encefalopatías y Enfermedades Transmisibles Emergentes, Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain;2. Unidad de Microbiología e Inmunología, Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain;3. Departamento de Anatomía, Embriología y Genética Animal, Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain;4. Departamento de Patología Animal, Hospital Veterinario, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain;1. Department of Animal Science, University of Minnesota, St. Paul, MN;2. Department of Animal and Range Science, Montana State University, Bozeman, MT;3. Department of Applied Economics, University of Minnesota, St. Paul, MN;1. Cuerpo Académico de Zoonosis y Enfermedades Emergentes, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Escobedo, Nuevo León, México;2. Epidemiología Veterinaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Escobedo, Nuevo León, México;3. Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada;1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA;2. Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Ames, IA |
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| Abstract: | The aim of the present study was to assess the effect of manganese (III) meso-tetrakis (4-benzoic acid) porphyrin (Mn-TBAP) on stallion sperm quality during storage at 5°C. In the present study, 18 ejaculates from three stallions were collected and diluted by INRA82 extender containing 0 (Mn-0), 100 (Mn-100), 200 (Mn-200), and 300 (Mn-300) μM of Mn-TBAP. Sperm motility, plasma membrane integrity and functionality, and lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa of diluted semen were evaluated in vitro at 2, 24, and 48 hours after storage at 5°C. The results showed that all evaluated sperm parameters, except MDA concentration, decreased significantly during the storage period. Total and progressive motility of spermatozoa were higher in Mn-200 extender (46.75 ± 0.58 and 27.62 ± 0.6, respectively) compared with Mn-0 (44.43 ± 0.58 and 25.13 ± 0.6, respectively) and Mn-300 (43.95 ± 0.58 and 25.28 ± 0.6, respectively) after 48 hours of storage at 5°C (P < .05). In addition, sperm plasma integrity and functionality were higher in Mn-200 extender (53.12 ± 0.6 and 46.63 ± 0.78, respectively) compared with Mn-0 (47.74 ± 0.6 and 40.96 ± 0.78, respectively), Mn-100 (48.63 ± 0.6 and 41.99 ± 078, respectively), and Mn-300 (46.11 ± 0.6 and 3.75 ± 0.78, respectively) after 48 hours of storage at 5°C (P < .05). The result showed also that MDA level was lower in Mn-100 extender (3.91 ± 0.06) compared with Mn-0 (4.51 ± 0.06), Mn-200 (4.25 ± 0.06), and Mn-300 (4.75 ± 0.06) after 48 hours of storage at 5°C (P < .05). In conclusion, INRA82 extender supplemented with 200-μM Mn-TBAP could efficiently preserve Caspian stallion spermatozoa after 48 hours of storage at 5°C. |
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| Keywords: | Stallion Cooling Mn-TBAP Spermatozoa |
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