| 不同地区稻虾综合种养系统的微生物群落结构分析 |
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| 引用本文： | 贾丽娟,王广军,夏耘,张凯,谢骏,郁二蒙,李志斐,龚望宝,田晶晶.不同地区稻虾综合种养系统的微生物群落结构分析[J].水产学报,2023,47(6):069107-069107. |
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| 作者姓名： | 贾丽娟 王广军 夏耘 张凯 谢骏 郁二蒙 李志斐 龚望宝 田晶晶 |
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| 作者单位： | 上海海洋大学;中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所 |
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| 基金项目： | 国家重点研发计划(2019YFD0900304)、广东省促进经济发展专项资金(2019B2)；中国水产科学研究院院级基本科研业务费(2021SJ-XC2),中国-东盟海上基金(CAMC-2018F) 第一作者：贾丽娟,硕士研究生,研究方向为水产动物健康养殖,E-mail：1661280002@qq.com通信作者：王广军,E-mail：wgj5810@163.com ,2,王广军2*,夏 耘2,张 凯2,谢骏2,郁二蒙2,李志斐2,龚望宝2,田晶晶2 |
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| 摘 要： | 为研究不同地区稻虾综合种养系统的环境及克氏原螯虾肠道的细菌群落结构差异，为改进不同地区稻虾综合养殖策略提供依据，采用Illumina Miseq高通量测序技术，研究了武汉、永州和韶关地区稻田养殖克氏原螯虾的水体、底泥及虾肠道细菌群落结构，并对水体、肠道菌群与环境因子之间的关系进行了分析。结果显示，武汉地区稻虾综合种养系统的水体、底泥及克氏原螯虾肠道细菌群落的多样性均大于永州地区和韶关地区。武汉地区的稻虾综合种养系统的水体及底泥的细菌群落结构与永州地区和韶关地区均相似，其中水体的优势菌门均为放线菌门、蓝细菌门、变形菌门和拟杆菌门；底泥的优势菌门均为变形菌门。武汉地区的克氏原螯虾肠道的优势菌门为变形菌门、厚壁菌门和拟杆菌门；优势菌属为柠檬酸杆菌属(Citrobacter,10.85%)、气单胞菌属(Aeromonas,9.88%)和Anaerorhabdus]＿furcosa＿group (8.43%)等。永州地区的克氏原螯虾肠道的优势菌门为厚壁菌门和放线菌门；优势菌属为ZOR0006 (9.78%)、拟杆菌属(Bacteroides,5.41%)和Anaerorhabdus]＿fur...
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| 关 键 词： | 克氏原螯虾 稻虾综合种养 水体 底泥 肠道 菌群结构 高通量测序 |
| 收稿时间： | 2021/5/6 0:00:00 |
| 修稿时间： | 2021/6/23 0:00:00 |
Analysis of microbial community structure in rice-shrimp integrated culture system of three different areas |
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| JIA Lijuan,WANG Guangjun,XIA Yun,ZHANG Kai,XIE Jun,YU Ermeng,LI Zhifei,GONG Wangbao,TIAN Jingjing.Analysis of microbial community structure in rice-shrimp integrated culture system of three different areas[J].Journal of Fisheries of China,2023,47(6):069107-069107. |
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| Authors: | JIA Lijuan WANG Guangjun XIA Yun ZHANG Kai XIE Jun YU Ermeng LI Zhifei GONG Wangbao TIAN Jingjing |
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| Institution: | National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University;China;Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences;Guangdong,China,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences |
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| Abstract: | Environmental microbiota plays important roles in the intestinal microbiota of aquatic animals. Procambarus clarkii has become the most important commercial species of shrimp in China with high commercial value, and the main culture mode of P. clarkii is rice-crayfish co-existing. P. clarkii culture is an important economic mainstay in Hubei province, China. However, information on the gut microbiota of P. clarkii is limited. To study the difference of bacterial community structure in the intestinal tract of P. clarkii and the environment of rice- crayfish comprehensive culture system in different areas, and to provide some basis for improving the comprehensive culture strategy of rice in different areas, Illumina MiSeq high-throughput sequencing method was used to sequence and analyze the two high-variation regions (V3-V4) of 16S rRNA gene of microbia of the water, sediment and intestine of P. clarkii in different regions (Wuhan, Yongzhou and Shaoguan). Redundancy analysis (RDA)was used to analyze the relationship among water, intestinal microorganisms and environmental factors. The results showed that the diversity of the bacterial community in the water, sediment and crawfish gut of rice shrimp integrated culture system in Wuhan area was higher than those in Yongzhou area and Shaoguan area. The bacterial community structure in the water and sediment of the rice shrimp integrated breeding system in Wuhan area was similar to those in Yongzhou area and Shaoguan area. Actinobacteria, Cyanobacteria, Proteobacteria and Bacteroidetes were the dominant phylum in water, Proteobacteria was the dominant phylum in sediments. The dominant bacteria in the intestin of P. clarkii in Wuhan area were Proteobacteria, Firmicutes and Bacteroidetes at the phylum level, and Citrobacter (10.85%), Aeromonas (9.88%) and Anaerorhabdus]_furcosa_group (8.43%) at the genus level. The dominant bacteria in the intestin of P. clarkii in Yongzhou area were Firmicutes and Actinomycetes at the phylum level, and ZOR0006(9.78%), Bacteroides (5.41%) and Anaerorhabdus]_furcosa_group (3.98%) at the genus level. The dominant bacteria in the intestine of P. clarkii in Shaoguan area were Firmicutes and Proteobacteria at the phylum level, and Citrobacter (9.31%) , bacteroidetes (4.29%) and Anaerorhabdus]_furcosa_group (4.58%) at the genus level. The results of correlation analysis between environmental factors and bacterial communities showed that TSS and TP had significant effect on the bacterial community structure of water, and PO43--P and CODcr had significant effect on the intestinal microbial community structure. The results show that there are some differences among the gut of P. clarkii, water and sediment of different regions, and the microbial structure of water bodies and sediment is more similar. The microbial diversity in the water body of rice shrimp integrated culture system in Wuhan area is the highest, which is beneficial to the stability of the breeding system.The culture environment in different regions did not affect the core phyla of microorganisms in the culture system, but changed the relative abundance of dominant phyla and genera. The nutrient content of culture environment will affect the microbial community structure to a certain extent. |
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| Keywords: | integrated shrimp in rice field water sediment intestine microbial community structure high-throughput sequencing |
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