| 猪圆环病毒2型高免卵黄抗体的研制与应用 |
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| 引用本文： | 索江华,吴玉臣,唐光武,郭爽,张桂云.猪圆环病毒2型高免卵黄抗体的研制与应用[J].中国畜牧兽医,2010,37(6):185-187. |
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| 作者姓名： | 索江华 吴玉臣 唐光武 郭爽 张桂云 |
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| 作者单位： | (1．郑州牧业工程高等专科学校， 郑州 450011； 2．河南农业大学， 郑州 450002) |
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| 基金项目： | *，河南省科技厅重点科技攻关项目 |
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| 摘 要： | 为制备猪圆环病毒2型(porcine circovirus serotype2,PCV-2)高免卵黄抗体,并对其治疗效果进行研究,通过自制猪圆环病毒2型(PCV-2)灭活苗,免疫SPF产蛋鸡,收集高免蛋,制备PCV-2高免卵黄抗体,对高免卵黄抗体进行无菌检验、活体动物安全性检测,用琼脂扩散方法进行效价检验和人工感染保育猪治疗试验,并将其应用于临床实践,观察其治疗效果。结果抗体效价达到1∶64;人工感染治疗试验治愈率达100%;临床试验治愈率为96%,应用效果良好。
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| 关 键 词： | 猪圆环病毒2型 高免卵黄抗体 人工感染 临床试验 |
Study and Application the Hyperimmunized Yolk Antibodies of PCV-2 in Piglets |
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| SUO Jiang-hua,WU Yu-chen,TANG Guang-wu,GUO Shuang,ZHANG Gui-yun.Study and Application the Hyperimmunized Yolk Antibodies of PCV-2 in Piglets[J].China Animal Husbandry & Veterinary Medicine,2010,37(6):185-187. |
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| Authors: | SUO Jiang-hua WU Yu-chen TANG Guang-wu GUO Shuang ZHANG Gui-yun |
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| Institution: | (1. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China；2. Henan Agricultural University, Zhengzhou 450002, China) |
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| Abstract: | The hyperimmunized yolk antibodies against porcine circovirus serotype 2 (PCV-2) was obtained，and its cure effect was tested. After the hens were immunized with the inactivated vaccine of PCV-2 and eggs of hyperimmunity were collected, the hyperimmunized yolk antibodies against PCV-2 were prepared; the proof-test of live bacteria，safe experiment，antibody titer by double diffusion test and artificially infected piglets with the antigen were conducted; and the antibodies were used on the farms to evaluate the clinical effect. The antibody titer reached to 1∶64, the cure rate of artificial infection was 100% and the cure rate of clinic treatment test was 96%. The antibody was worth to be used in practice on the farms. |
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| Keywords: | PCV-2 hyperimmunized yolk antibodies infection of man-made practice |
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