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| 安祖花细菌性疫病的Nested-PCR检测 | |
| 引用本文： | 孟鹤,金茂勇,肖橘清,张宝珠,明军,袁素霞,刘春,张宁.安祖花细菌性疫病的Nested-PCR检测[J].园艺学报,2011,38(10):2017-2020. |
| 作者姓名： | 孟鹤 金茂勇 肖橘清 张宝珠 明军 袁素霞 刘春 张宁 |
| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081 2. 北京市大兴区苗圃,北京,102601 3. 华中农业大学园艺林学学院,武汉,430070 |
| 基金项目： | 中国农业科学院与北京市大兴区科技合作项目(YLHH200900106); 北京市科技计划研发攻关项目 |
| 摘 要： | 根据基因库中已发表的安祖花细菌性疫病致病菌粉黛黄单胞杆菌（Xanthomonas campestris pv.dieffenbachiae）基因序列设计引物XcF1/XcR1、XcF2/XcR2,通过扩增条件优化,建立了安祖花细菌性疫病的Nested-PCR检测方法,可以直接从感染细菌性疫病的安祖花组织DNA中扩增出1... |
| 关 键 词： | 安祖花 细菌性疫病 粉黛黄单胞杆菌 Nested-PCR |
Detection of Xanthomonas campestris pv.dieffenbachiae on Anthurium andraeanum by Nested-PCR |
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| MENG He ,JIN Mao-yong ,XIAO Ju-qing ,ZHANG Bao-zhu ,MING Jun ,YUAN Su-xia ,LIU Chun , ZHANG Ning.Detection of Xanthomonas campestris pv.dieffenbachiae on Anthurium andraeanum by Nested-PCR[J].Acta Horticulturae Sinica,2011,38(10):2017-2020. | |
| Authors: | MENG He JIN Mao-yong XIAO Ju-qing ZHANG Bao-zhu MING Jun YUAN Su-xia LIU Chun ZHANG Ning |
| Institution: | 1. Laboratory of Tumor Biochemistry, Wannan Medical College, Wuhu 241002, China; 2. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China |
| Abstract: | A Nested-PCR method was developed to detect the bacterial pathogens of Anthurium andraeanum by using two sets of specific primers designed according to Xanthomonas campestris pv.dieffenbachiae gene sequences.All samples infected by Xanthomonas campestris pv.dieffenbachiae could be amplified by Nested-PCR,yielding one special band with size of 343 bp.The amplified sequences were presented in the Xanthomonas campestris pv.dieffenbachiae sequence X70380.1 in GenBank.Xanthomonas campestris pv.dieffenbachiae was... |
| Keywords: | Anthurium andraeanum bacterial blight Xanthomonas campestris pv dieffenbachiae Nested-PCR |
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