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| 应用real-time RT-PCR监测柑橘衰退病毒强、弱毒株的时序变化 | |
| 引用本文： | 邹勤,周彦,李中安,周常勇,刘永清,苏华楠.应用real-time RT-PCR监测柑橘衰退病毒强、弱毒株的时序变化[J].园艺学报,2011,38(11):2193-2198. |
| 作者姓名： | 邹勤 周彦 李中安 周常勇 刘永清 苏华楠 |
| 作者单位： | 1. 中国农业科学院柑桔研究所国家柑桔工程技术研究中心,重庆400712／西南大学植物保护学院,重庆400716 2. 中国农业科学院柑桔研究所国家柑桔工程技术研究中心,重庆,400712 3. 西南大学植物保护学院,重庆,400716 |
| 基金项目： | 国家自然科学基金项目(30771485); 教育部创新团队项目(IRT0976); 行业公益项目(200903004); 重庆市自然科学基金项目(2008BB1270) |
| 摘 要： | 使用褐色橘蚜在提前7个月预免疫接种了柑橘衰退病毒（Citrus tristeza virus，CTV）弱毒株CT11的锦橙植株上拮抗接种强毒株CT14，应用real-time RT-PCR技术，监测供试植株中CTV强、弱毒株的含量变化，同时采用内参基因18S rRNAF/R及nad5F/R校正检测结果。试验结果表明，在拮抗接种3个月内始终未能在预免疫的拮抗植株中检测出强毒株CT14。在25头蚜虫拮抗接种后45 d和50头蚜虫拮抗接种后10 d，绝大多数预免疫的拮抗植株中弱毒株的含量高于预免疫的负对照植株，此后弱毒株的含量基本下降到负对照植株的水平，而负对照植株内弱毒株的含量变化不明显。 |
| 关 键 词： | 柑橘 柑橘衰退病毒 real-time RT-PCR 弱毒株交叉保护 |
Monitoring the Temporal Change of the Mild and Severe Strain of Citrus tristeza virus by Real-time RT-PCR |
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| ZOU Qin,ZHOU Yan,LI Zhong-an,ZHOU Chang-yong,LIU Yong-qing, SU Hua-nan.Monitoring the Temporal Change of the Mild and Severe Strain of Citrus tristeza virus by Real-time RT-PCR[J].Acta Horticulturae Sinica,2011,38(11):2193-2198. | |
| Authors: | ZOU Qin ZHOU Yan LI Zhong-an ZHOU Chang-yong LIU Yong-qing SU Hua-nan |
| Institution: | ZOU Qin1,2,ZHOU Yan1,LI Zhong-an1,ZHOU Chang-yong1,LIU Yong-qing2,and SU Hua-nan2 (1National Citrus Engineering Research Centre,Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712,China,2College of Plant Protection,Southwest University,Chongqing 400716,China) |
| Abstract: | Jincheng sweet orange seedlings were pre-inoculated with CTV mild isolate CT11 by grafting,and seven months later,challenged by severe CTV isolate CT14 with Toxoptera citricida. To identify and quantitate the mild and severe CTV isolates within the same plant,a real-time RT-PCR technique was modified and housekeeping genes 18S rRNAF/R and nad5F/R were approached for rectification. The results showed that CT14 was not detectable in any challenge-inoculated plants three months after CT14 attacked through aphi... |
| Keywords: | Citrus Citrus tristeza virus real-time RT-PCR mild strain cross protection |
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