| 侵染节瓜的3种病毒多重PCR检测体系的建立 |
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| 引用本文： | 赵芹,李华平,谢大森,罗少波,彭庆务.侵染节瓜的3种病毒多重PCR检测体系的建立[J].园艺学报,2011,38(11):2215-2222. |
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| 作者姓名： | 赵芹 李华平 谢大森 罗少波 彭庆务 |
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| 作者单位： | 1. 广东省农业科学院蔬菜研究所,广州,510640
2. 华南农业大学植物病毒研究室,广州,510642 |
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| 基金项目： | 广东省植物保护新技术重点实验室开放基金项目(植重2010-3) |
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| 摘 要： | 根据侵染节瓜3种病毒——小西葫芦黄花叶病毒（ZYMV）、番木瓜环斑病毒（PRSV）和黄瓜花叶病毒（CMV）的外壳蛋白基因保守区序列设计特异引物，在建立各种病毒单项PCR技术的基础上，通过优化多重PCR反应条件，初步建立了能同步、快速、灵敏地检测这3种病毒的多重PCR检测体系，为节瓜病毒病原的检测提供了理论与技术支持。
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| 关 键 词： | 节瓜 小西葫芦黄花叶病毒 番木瓜环斑病毒 黄瓜花叶病毒 多重PCR |
Development of a Multiplex RT-PCR Protocol for the Detection of Three Viruses on Benincasa hispida. var. chieh-qua |
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| ZHAO Qin,LI Hua-ping,XIE Da-sen,LUO Shao-bo, PENG Qing-wu.Development of a Multiplex RT-PCR Protocol for the Detection of Three Viruses on Benincasa hispida. var. chieh-qua[J].Acta Horticulturae Sinica,2011,38(11):2215-2222. |
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| Authors: | ZHAO Qin LI Hua-ping XIE Da-sen LUO Shao-bo PENG Qing-wu |
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| Institution: | ZHAO Qin1,LI Hua-ping2,XIE Da-sen1,LUO Shao-bo1,and PENG Qing-wu1 (1Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China,2Laboratory of Plant Virology,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | According to the conserved nucleotide sequences of coat protein genes of Zucchini yellow mosaic virus(ZYMV),Papaya ring spot virus(PRSV)and Cucumber mosaic virus(CMV)infected chieh-qua specific primers were designed. Based on the establishment of the optimal RT-PCR for the single detection of ZYMV,PRSV and CMV,a multiplex RT-PCR protocol for the detection of three viruses on chieh-qua was developed,which provided a simple,rapid and economic method for simultaneous detection of three chieh-qua viruses to sup... |
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| Keywords: | chieh-qua Benincasa hispida(Thunb )Cogn var chieh-qua How Zucchini yellow mosaic virus Papaya ring spot virus Cucumber mosaic virus multiplex RT-PCR |
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