An Assessment of the Genetic Diversity within a Collection of Wild Cranberry (<Emphasis Type="Italic">Vaccinium macrocarpon</Emphasis> Ait.) Clones with RAPD-PCR |
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| Authors: | S C Debnath |
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| Institution: | (1) Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, 308 Brookfield Road, St. John's, P.O. Box 39088, Newfoundland and Labrador, A1E 5Y7, Canada |
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| Abstract: | Forty-three wild cranberry (Vaccinium macrocarpon Ait.) clones collected from four Canadian provinces and five cranberry cultivars were assessed for genetic variability by
using random amplified polymorphic DNA (RAPD)-PCR. Fourteen primers generated 161 polymorphic RAPD-PCR bands. A substantial
degree of genetic diversity was found among the wild cranberry collections. Cluster analysis by the unweighted pair-group
method with arithmetic averages (UPGMA) separated the wild clones and three cultivars into five main clusters, and identified
the two remaining cultivars as outliers. Furthermore, within four clusters, the genotypes tended to form sub-clusters that
were in agreement with the principal coordinate (PCO) analysis. Geographical distribution explained 10% of total variation
as revealed by analysis of molecular variance (AMOVA). The RAPD markers detected a sufficient degree of polymorphism to differentiate
among cranberry clones and cultivars, making this technology valuable for germplasm management and the more efficient choice
of parents in current cranberry breeding programs. |
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| Keywords: | DNA fingerprinting Molecular marker Multivariate analysis Vaccinium macrocarpon |
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