HS-protein associates in the aqueous/oil system: composition and colloidal properties |
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Authors: | Maria G Chernysheva Gennadii A Badun |
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Institution: | 1. Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia
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Abstract: | Purpose Despite experiments with humic substances and positively charged proteins, the colloidal behavior of HS-protein mixture in the system of two immiscible liquids has been neglected. In this context, the main objective of this study was to reveal the interference of HS and globular proteins on its partition in an aqueous/organic liquid system and the adsorption at liquid/liquid interface as a model of natural organic matter interaction with proteins in nature at hydrophobic/hydrophilic surfaces. Materials and methods Coal humic acids (HA) and two globular proteins lysozyme and albumin were under the test. Aqueous phase was prepared in phosphate-buffered saline (pH 7.2?±?0.1, 0.16 M); p-xylene was chosen as an organic phase. Experiments were performed for fixed concentration of protein (0.1 g L?1 for lysozyme and 0.06 g L?1 for albumin) and varied HA concentration from 0.2 to 50 mg L?1. Radiotracer method including tritium thermal activation and scintillation phase method, dynamic light scattering, and optical microscopy were used to control mixed adsorption layer at the aqueous/p-xylene interface and composition of each contact phase. Results and discussion The results suggest that if both HA and protein are negatively charged (HA-albumin mixture), the mechanism of interaction between them in the bulk of water and at liquid/liquid interface is controlled by HA concentration. At low HA concentrations, free protein prevents HA adsorption at liquid/liquid interface and its transition to the organic phase via coulomb repulsion. At high HA concentration, the formation of hydrophilic complexes occurs via both electrostatic attraction between positively charged amino acid residues and the hydrophobic interaction. In HA-lysozyme mixture, the interaction between protein and HA is preferably provided by electrostatic attraction that provides higher hydrophobicity of HA-lysozyme complex compared with free HA. An increase in HA concentration results in partial recharge of the conjugate that leads to lysozyme amount reduction at the interface. We also measured the composition of spontaneously formed precipitate of HA-lysozyme conjugate and followed its self-organization. Conclusions This work demonstrates colloidal chemical behavior of net positively and negatively charged model protein by coal humic acids under environmentally relevant solution conditions in the system of two immiscible liquids that were used as a model of natural membrane. For the first time, quantities of both protein and HA in mixed adsorption layer at the liquid/liquid interface in the cases of positively and negatively charged protein have been determined. |
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