| 长刺蒺藜草根际土壤解磷细菌群落的多样性分析 |
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| 引用本文： | 宋振,王然,高金会,付卫东,马涛,袁至立,张国良.长刺蒺藜草根际土壤解磷细菌群落的多样性分析[J].植物保护学报,2022,49(5):1358-1366. |
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| 作者姓名： | 宋振 王然 高金会 付卫东 马涛 袁至立 张国良 |
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| 作者单位： | 中国农业科学院农业环境与可持续发展研究所, 北京 100081 |
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| 基金项目： | 国家自然科学基金面上项目（41977203），国家重点研发计划（2021YFD1400300，2022YFC2601401，2022YFC2601404） |
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| 摘 要： | 为揭示外来入侵植物长刺蒺藜草 Cenchrus longispinus能够在贫瘠沙性土壤中快速生长、定殖的入侵机制，利用传统功能微生物筛选培养法、 16S rDNA高通量测序技术及细菌基因组重复序列PCR（genome repetitive sequence PCR， rep-PCR）基因指纹图谱技术分析其根际土壤中解磷细菌的丰富度及多样性，并与本地伴生植物冰草Agropyron cristatum和狗尾草Setaria viridis根际土壤中的解磷细菌群落进行比较。结果显示，长刺蒺藜草根际土壤中解磷细菌的数量分别达到8.61×104 CFU/g和7.34×104 CFU/g，显著高于其余土壤样品。相较于空白对照土壤，长刺蒺藜草根际土壤中的解有机磷细菌数量增加最多，增加了87.0%，冰草和狗尾草根际土壤中的解有机磷细菌数量则分别增加了28.3%和15.2%；而长刺蒺藜草、冰草和狗尾草根际土壤中的解无机磷细菌数量分别增加了36.7%、 89.8%和24.5%。聚类分析结果表明，不同根际土壤中解磷细菌之间没有共有的优势聚类群。长刺蒺藜草根际土壤中2种解磷细菌群落的物种均匀度指数显著高于空白对照土壤和冰草及狗尾草根际土壤，而物种丰富度指数和Shannon-Wiener多样性指数均显著低于冰草及狗尾草根际土壤；其根际土壤中解无机磷细菌优势菌属经鉴定为金黄杆菌属 Chryseobacterium。表明长刺蒺藜草在贫瘠沙土中对磷素的高效利用可能是其快速入侵并定殖的机制之一。
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| 关 键 词： | 入侵植物 长刺蒺藜草 解无机磷细菌 解有机磷细菌 入侵机制 |
| 收稿时间： | 2022/9/9 0:00:00 |
Community diversity analysis of phosphorus solubilizing bacteria in rhizosphere of spiny burrgrass Cenchrus longispinus |
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| Song Zhen,Wang Ran,Gao Jinhui,Fu Weidong,Ma Tao,Yuan Zhili,Zhang Guoliang.Community diversity analysis of phosphorus solubilizing bacteria in rhizosphere of spiny burrgrass Cenchrus longispinus[J].Acta Phytophylacica Sinica,2022,49(5):1358-1366. |
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| Authors: | Song Zhen Wang Ran Gao Jinhui Fu Weidong Ma Tao Yuan Zhili Zhang Guoliang |
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| Institution: | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | In order to reveal the invasion mechanism of the invasive alien spiny burrgrass Cenchrus longispinus, which can rapidly grow and colonize in poor sandy soil, the richness and diversity of phosphorus solubilizing bacteria in its rhizosphere were analyzed by using traditional functional microorganism screening and culture method, 16S rDNA high-throughput sequencing technology and genomic repetitive sequence PCR (rep-PCR) technology, compared with those in the rhizosphere of the local associated plants Agropyron cristatum and Setaria viridis. The results showed that the number of phosphorus solubilizing bacteria in the rhizosphere of C. longispinus were significantly more than the other three treatments, reaching 8.61×104 CFU/g and 7.34×104 CFU/g for organic and inorganic phosphoric bacteria, respectively. Organic phosphorus bacteria in rhizosphere of C. longispinus increased by 87.0%, and increased 28.3% and 15.2% in rhizosphere of A. cristatum and S. viridis, respectively, compared with that of control; and the number of inorganic phosphorus bacteria in the rhizosphere of C. longispinus, A. cristatum and S. viridis increased by 36.7%, 89.8% and 24.5%, respectively compared with the control.There was no common dominant cluster among the four treatments. The species evenness index of phosphate-solubilizing microorganisms in the rhizosphere of C. longispinus was significantly higher than those of the other three treatments, while the species richness index and Shannon-Wiener diversity index were significantly lower than those of A. cristatum and S. viridis. The dominant inorganic phosphorus bacterial groups in the rhizosphere of C. longispinus were identified as Chryseobacterium. This study indicated that the efficient use of phosphorus by C. longispinus in poor sandy soil may be one of the mechanisms of its rapid invasion and colonization. |
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| Keywords: | invasive plant Cenchrus longispinus inorganic phosphorus bacteria organic phosphorus bacteria invasive mechanism |
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