| 玉米茉莉酸信号途径参与抵抗玉米褪绿斑驳病毒的侵染 |
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| 引用本文： | 刘学东,刘思佳,陈曦,范在丰,周涛.玉米茉莉酸信号途径参与抵抗玉米褪绿斑驳病毒的侵染[J].植物保护学报,2022,49(5):1342-1348. |
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| 作者姓名： | 刘学东 刘思佳 陈曦 范在丰 周涛 |
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| 作者单位： | 中国农业大学植物保护学院, 农业农村部植物检疫性有害生物监测防控重点实验室, 北京 100193 |
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| 基金项目： | 国家重点研发计划（2018YFD020062），财政部和农业农村部国家现代农业产业技术体系（CARS-02） |
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| 摘 要： | 为研究茉莉酸（jasmonic acid， JA）信号途径在玉米响应玉米褪绿斑驳病毒（maize chloroticmottle virus， MCMV）侵染中的作用，利用外源喷施茉莉酸甲酯 （methyl jasmonate， MeJA）方法，采用病毒诱导的基因沉默技术以及玉米原生质体过表达探究JA信号途径是否参与玉米抗MCMV侵染。结果表明，相比于对照， MCMV在外源喷施MeJA的玉米植株上引起的褪绿和花叶症状明显减轻， MCMV基因组RNA积累水平下降了69%，外壳蛋白 （coat protein， CP）积累水平下降了43%，表明MeJA处理提升了玉米植株对MCMV的抗性。在沉默JA信号途径抑制基因 ZmJAZ5的玉米植株上，相比于对照植株， MCMV引起的褪绿及花叶症状也明显减轻， MCMV的基因组RNA积累水平下降了71%， CP积累水平下降了56%。在玉米原生质体中过表达ZmJAZ5后，与对照相比， MCMV基因组RNA积累水平上升了1.58倍， CP积累水平上升了1.34倍。表明JA信号途径在玉米抵抗MCMV侵染过程中发挥着关键作用。
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| 关 键 词： | 玉米褪绿斑驳病毒 激素信号 茉莉酸 病毒诱导的基因沉默 |
| 收稿时间： | 2022/9/13 0:00:00 |
Jasmonic acid signaling confers resistance to maize chlorotic mottle virus in maize |
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| Liu Xuedong,Liu Siji,Chen Xi,Fan Zaifeng,Zhou Tao.Jasmonic acid signaling confers resistance to maize chlorotic mottle virus in maize[J].Acta Phytophylacica Sinica,2022,49(5):1342-1348. |
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| Authors: | Liu Xuedong Liu Siji Chen Xi Fan Zaifeng Zhou Tao |
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| Institution: | Key Laboratory of Surveillance and Management for Plant Quarantine Pests of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193, China |
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| Abstract: | To study the function of jasmonic acid (JA) signaling in maize response to maize chlorotic mottle virus (MCMV) infection, spraying of methyl jasmonate (MeJA), virus-induced gene silencing and over-expression in maize protoplasts were conducted to explore whether JA signaling correlates with resistance to MCMV. The results showed that, compared to controls, MCMV caused milder chlorotic and mosaic symptoms on maize plants sprayed with MeJA. After treatment, the accumulation lev els of MCMV genomic RNA and coat protein (CP) decreased by 69% and 43%, respectively, suggesting enhanced maize resistance to MCMV. Similarly, on plants of silencing ZmJAZ5, a JA-signaling suppression gene, milder chlorotic and mosaic symptoms were observed following MCMV infection, while ac cumulation levels of MCMV genomic RNA decreased 71%, and CP levels decreased 56% compared with that on control plants. Moreover, overexpression of ZmJAZ5 in maize protoplasts up-regulated 1.58- and 1.34-fold of MCMV genomic RNA and CP accumulation levels, respectively. The results indicated that JA signaling played a crucial role in maize resistance to MCMV infection. |
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| Keywords: | maize chlorotic mottle virus (MCMV) hormonal signaling jasmonic acid (JA) virus-in duced gene silencing (VIGS) |
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