Plasma biomarkers profile of female dogs with mammary carcinoma and its association with clinical and pathological features |
| |
| Authors: | R P Soares L G R Ribeiro K A Damasceno A T Costa A Teixeira‐Carvalho O A Martins‐Filho G D Cassali |
| |
| Institution: | 1. Laboratório de Biomarcadores de Diagnóstico e Monitora??o, Centro de Pesquisas René Rachou, Funda??o Oswaldo Cruz, Belo Horizonte, MG, Brazil;2. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;3. Laboratório de Patologia Comparada (LPC), Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;4. Departamento de Patologia e Clínica, Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, BA, Brazil |
| |
| Abstract: | The immunological biomarkers profiles were evaluated using Luminex as putative measures to monitor canine mammary carcinomas (MCs). Forty female dogs were categorized into benign mixed tumour (MC‐BMT = 28) and mammary carcinoma (MC=12). The ascendant biomarker signatures were used to compare the groups. For example, a higher frequency of MC‐BMT animals producing IL‐6, CXCL‐8 and CXCL‐10 was observed, whereas for the MC group IL‐2 and CXCL‐8 were detected. MC‐BMT animals without metastasis had an increase in the levels of IL‐2, CXCL‐8, CXCL‐10, IL‐6, TNF‐α, IL‐15 and a decrease in IL‐10 and CXCL‐8. MC‐BMT animals with metastasis showed only an increase in CXCL‐10 and a decrease in IL‐18. After comparing the ascendant signatures following the presence of metastasis in both groups, a higher frequency of dogs exhibiting IL‐10 production was observed. Pearson correlation (P = 0.0273) and receiver operating characteristic (ROC) curve analysis revealed that this pattern was associated with worse outcome and lower survival rates in MC animals. |
| |
| Keywords: | clinical pathology comparative oncology immunology metastasis oncology |
|
|
