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| 四溴双酚A对土壤无机氮转化的影响及其微生物学机理初探 | |
| 引用本文： | 张博伟,王海静,宋茂勇,谢慧君,张建.四溴双酚A对土壤无机氮转化的影响及其微生物学机理初探[J].浙江农业学报,2019,31(4):639. |
| 作者姓名： | 张博伟 王海静 宋茂勇 谢慧君 张建 |
| 作者单位： | 1.山东大学 环境研究院,山东 济南 250100;2.山东大学 环境科学与工程学院,山东 济南 250100;3.中国科学院 生态环境研究中心,北京 100085 |
| 基金项目： | 中国科学院战略重点研究项目(XB14010300);微生物技术国家重点实验室开放课题(M2015-07) |
| 摘 要： | 四溴双酚A(TBBPA)作为一种新型污染物,一旦进入土壤并在土壤中积聚,将会影响土壤物质循环及相关微生物活性。在实验室模拟条件下,研究了不同浓度(4~40 mg·kg-1)TBBPA对土壤中铵态氮($NH^{+}_{4}-N$)、亚硝态氮($NO^{-}_{2}-N$)和硝态氮($NO^{-}_{3}-N$)转化的影响,利用高通量测序测定了TBBPA对无机氮转化菌群结构的变化,并推测其可能影响的代谢途径。结果表明,40 mg·kg-1的TBBPA对土壤无机氮循环有显著(P<0.05)影响,其在好氧条件下增加了硝化和反硝化菌的丰度,促进了$NH^{+}_{4}-N$和$NO^{-}_{3}-N$的转化,在厌氧条件下反硝化细菌数量减少,抑制了$NO^{-}_{3}-N$的转化。40 mg·kg-1 TBBPA不论是在厌氧还是好氧条件下,都增加了土壤中变形菌门(Proteobacteria)的丰度,降低了绿弯菌门(Chloroflexi)和放线菌门(Actinobacteria)的丰度。通过预测氮转化基因功能看出, 40 mg·kg-1 TBBPA处理在好氧条件下通过增强narG和nirK基因的表达有助于提高$NO^{-}_{3}-N$的转化,在厌氧条件下通过限制nasB的表达会抑制$NO^{-}_{3}-N$的转化。 |
| 关 键 词： | 土壤微生物 无机氮转化 高通量测序 四溴双酚A |
| 收稿时间： | 2018-09-21 |
Effect of tetrabromobisphenol A on inorganic nitrogen transformation in soil and its possible microbiological mechanism |
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| ZHANG Bowei,WANG Haijing,SONG Maoyong,XIE Huijun,ZHANG Jian.Effect of tetrabromobisphenol A on inorganic nitrogen transformation in soil and its possible microbiological mechanism[J].Acta Agriculturae Zhejiangensis,2019,31(4):639. | |
| Authors: | ZHANG Bowei WANG Haijing SONG Maoyong XIE Huijun ZHANG Jian |
| Institution: | 1. Environment Research Institute, Shandong University, Jinan 250100, China; 2. School of Environmental Science and Engineering, Shandong University, Jinan 250100, China; 3. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
| Abstract: | As one of the emerging contaminants, tetrabromobisphenol A (TBBPA) could accumulate in the soil and affect soil materials cycling as well as soil microbes. TBBPAs with different concentrations (4-40 mg·kg-1) were added in soil to investigate their effects on $NH^{+}_{4}-N$, $NO^{-}_{2}-N$ and $NO^{-}_{3}-N$ transformation in lab. The bacterial community structures, as well as the prediction of the functions related to inorganic nitrogen transformation influenced by TBBPA, were analyzed with Illumina sequencing. It was shown that 40 mg·kg-1 TBBPA had a significant (P<0.05) effect on soil inorganic nitrogen transformation. Addition of 40 mg·kg-1 TBBPA increased the abundance of nitrifying bacteria and denitrifying bacteria and promoted $NH^{+}_{4}-N$ and $NO^{-}_{3}-N$ transformation in the aerobic condition, while decreased the abundance of denitrifying bacteria and inhibited $NO^{-}_{3}-N$ transformation in anaerobic condition. In addition, 40 mg·kg-1 TBBPA also had remarkable effect on microbial community structure, including a positive effect on the relative abundances of Proteobacteria and a negative effect on the relative abundances of Chloroflexi and Actinobacteria. By predicting the microbial functions related to nitrogen transformation, it could be inferred that 40 mg·kg-1 TBBPA induced the expression of narG and nirK and promoted $NO^{-}_{3}-N$ transformation in the aerobic condition, yet reduced the expression of nasB and inhibited $NO^{-}_{3}-N$ transformation in anaerobic condition. |
| Keywords: | soil microbes inorganic nitrogen transformation high-throughput sequencing tetrabromobisphenol A |
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