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| 白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析 | |
| 引用本文： | 徐小钦,杜雪,李国勤,张龙,陶争荣,卢立志.白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析[J].浙江农业学报,2019,31(5):704. |
| 作者姓名： | 徐小钦 杜雪 李国勤 张龙 陶争荣 卢立志 |
| 作者单位： | 1.浙江省农业科学院 畜牧兽医研究所,浙江 杭州310021;2.西华师范大学 生态研究院,四川 南充637000;3.南京农业大学 动物科技学院,江苏 南京 210095;4.农业农村部农产品信息溯源重点实验室,浙江 杭州 310021 |
| 基金项目： | 浙江省农业(畜禽)新品种选育重大科技专项(2016C02054-16);浙江省农业科学院地方科技合作项目(WZ2017002) |
| 摘 要： | 旨在获得白羽王鸽(Columba livia)卵清蛋白关联蛋白Y基因(OVALY)的cDNA序列,并进行生物信息分析。研究结果显示:OVALY基因cDNA全长为2 068 bp,其中5'非编码区序列180 bp,3'非编码区序列720 bp,开放阅读框1 164 bp,编码的蛋白分子量约44.19 ku,含氨基酸残基388个(GenBank∶KX230792);与NCBI数据库中朱鹮(Nipponianippon)OVALY基因序列同源性最高(81%),与白尾鹰(Haliaeetusalbicilla)和沙鸡(Pteroclesgutturalis)OVALY基因序列的同源性分别为80%和79%。经潜在糖基化位点和磷酸化位点预测分析发现,OVALY蛋白结构中潜在糖基化位点和磷酸化位点分别为4个和22个;经CDD(conserved domain database)数据库分析发现,OVALY蛋白具有丝氨酸蛋白酶抑制剂家族的反应中心区域,属于丝氨酸蛋白酶抑制剂超家族。 |
| 关 键 词： | 白羽王鸽 卵清蛋白关联蛋白Y( |
| 收稿时间： | 2018-09-28 |
Molecular cloning and bioinformatics analysis of white king pigeon (Columba livia) ovalbumin-related protein Y gene |
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| XU Xiaoqin,DU Xue,LI Guoqin,ZHANG Long,TAO Zhengrong,LU Lizhi.Molecular cloning and bioinformatics analysis of white king pigeon (Columba livia) ovalbumin-related protein Y gene[J].Acta Agriculturae Zhejiangensis,2019,31(5):704. | |
| Authors: | XU Xiaoqin DU Xue LI Guoqin ZHANG Long TAO Zhengrong LU Lizhi |
| Institution: | 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. Institute of Ecology, China West Normal University, Nanchong 637000, China; 3. College of Animal Sciences & Technology, Nanjing Agricultural University, Nanjing 210095, China;; 4.Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou 310021,China |
| Abstract: | The experiment was conducted to clone the whole cDNA of OVALY gene in Columba liviaand analyze the bioinformatic information of the cDNA sequence.The nucleotide sequence of a full-length 2 068 bp cDNA for OVALY was obtained, containing 5'UTR (180 bp), CDS (1 164 bp) and 3'UTR (720 bp), and coding 388 amino acids (44.19 ku, GenBank accession number: KX230792). The OVALY cDNA sequence of white king pigeon was most similar to Nipponianippon, and the homology was 81%. This sequence was also similar to Haliaeetusalbicilla and Pteroclesgutturalis, and the homologies were 80% and 79%, respectively. 4 N-glycosylated sites and 22 phosphorylation sites were detected in these amino acids. Conserved domain analysis of OVALY protein showed this protein had a conserved domain for serpin superfamily. |
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