| 短枝木麻黄幼苗对低温胁迫的生理响应 |
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| 引用本文： | 李楠,李贺鹏,江波,叶碧欢,陈友吾,李海波.短枝木麻黄幼苗对低温胁迫的生理响应[J].浙江农林大学学报,2019,36(4):678-686. |
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| 作者姓名： | 李楠 李贺鹏 江波 叶碧欢 陈友吾 李海波 |
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| 作者单位： | 浙江省林业科学研究院, 浙江 杭州 310023 |
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| 基金项目： | 浙江省农业(林木)新品种选育重大科技专项2016C02056-9 |
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| 摘 要： | 以短枝木麻黄Casuarina equisetifolia耐寒无性系ZS7和不耐寒无性系HN1幼苗为供试材料，在人工气候箱内采用基质栽培方式，研究在-2，-5，-8，-11 ℃共4个温度梯度胁迫2 h以及-5 ℃持续胁迫1，2，5，8，16，24，48，72 h后2种无性系幼苗相关生理指标的变化趋势以及耐寒性差异，探讨短枝木麻黄适应低温环境的生理机制。结果表明:在低温梯度胁迫下，耐寒无性系的过氧化氢（H2O2）和丙二醛（MDA）质量摩尔浓度的增幅小于不耐寒无性系；叶绿素、脯氨酸（Pro）和可溶性蛋白质质量分数，超氧化物歧化酶（SOD），过氧化物酶（POD），过氧化氢酶（CAT），抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）活性，以及还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）质量摩尔浓度的降幅均小于不耐寒无性系。耐寒无性系的GSH/GSSG比值呈上升的趋势，而不耐寒无性系的呈先下降后上升趋势。在-5 ℃持续胁迫下，2种无性系各生理指标呈现波动变化，增幅（降幅）与到达峰值时间不同。无论是在低温梯度胁迫还是在低温持续胁迫处理过程中，耐寒无性系的SOD，POD，CAT，APX和GR活性以及叶绿素、可溶性蛋白质和Pro质量分数、GSH质量摩尔浓度都显著高于不耐寒无性系。耐寒性不同的2种短枝木麻黄无性系耐寒的生理机制明显不同，耐寒性强的无性系通过在低温胁迫下保持较高的可溶性蛋白质和Pro等渗透调节物质质量分数，增强抗氧化酶活性，提高非酶抗氧化剂水平，抑制叶绿素质量分数的下降及膜脂过氧化程度的加剧，进而提高抗寒性来抵御低温。
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| 关 键 词： | 植物生理学 短枝木麻黄 低温胁迫 耐寒性 生理响应 |
| 收稿时间： | 2018-08-30 |
Physiological response to low temperature stress in Casuarina equisetifolia seedlings |
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| LI Nan,LI Hepeng,JIANG Bo,YE Bihuan,CHEN Youwu,LI Haibo.Physiological response to low temperature stress in Casuarina equisetifolia seedlings[J].Journal of Zhejiang A&F University,2019,36(4):678-686. |
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| Authors: | LI Nan LI Hepeng JIANG Bo YE Bihuan CHEN Youwu LI Haibo |
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| Institution: | Zhejiang Academy of Forestry, Hangzhou 310023, Zhejiang, China |
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| Abstract: | This study was conducted to explore changes in relevant physiological indexes of cold-tolerant and cold-intolerant Casuarina equisetifolia as well as physiological mechanisms to adapt to a low-temperature environment, and to provide a basis for further study of cold resistance in C. equisetifolia. Using a substrate culture, the related physiological indexes of cold-tolerant (ZS7) and cold-intolerant (HN1) clones of C. equisetifolia seedlings with successive low temperature stresses at -2, -5, -8, and -11 ℃ for 2 h, as well as precise expression patterns with low temperature stresses of -5 ℃ for 1, 2, 5, 8, 16, 24, 48, and 72 h in climate chambers were studied. Results showed that for successive low temperature stresses, the rising amplitude of H2O2 and malondialdehyde (MDA) content in the cold-tolerant clones were significantly lower (P < 0.05) than in the cold-tolerant clones. Basically, the decreased amplitude of total chlorophyll, soluble protein, proline contents, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and ascorbate peroxidase (APX), as well as contents of glutathione reductase (GR), glutathione (GSH), and oxidized glutathione (GSSG) in ZS7 were significantly lower (P < 0.05) than in HN1. The GSH/GSSG ratio in ZS7 increased gradually; whereas, HN1 decreased first and then increased. For low temperature stress at -5 ℃, the rising amplitude or decrease in amplitude of physiological indexes in the two clones were different as was the time to its peak for physiological indexes of SOD, POD, CAT, APX, and GR as well as contents of total chlorophyll, soluble protein, proline, and GSH with ZS7 being generally higher than HN1. Thus, different clones showed different physiological response mechanisms to low temperature stress with the cold-tolerant clone resisting low temperature and enhancing cold resistance by maintaining the content of soluble protein and proline, promoting antioxidant enzyme activities and antioxidant contents, decreasing the accumulation of H2O2 and MDA, and inhibiting chlorophyll deterioration. |
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