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| 基于TaqMan PCR鉴定猪源性成分的引物和探针研发 | |
| 引用本文： | 郭梁,李春冬,徐伟良,郭元晟.基于TaqMan PCR鉴定猪源性成分的引物和探针研发[J].浙江农业学报,2019,31(1):74. |
| 作者姓名： | 郭梁 李春冬 徐伟良 郭元晟 |
| 作者单位： | 1.锡林郭勒职业学院,内蒙古 锡林浩特 026000; 2.锡林郭勒生物工程研究院,内蒙古 锡林浩特 026000; 3.锡林郭勒食品检验检测和风险评估中心,内蒙古 锡林浩特 026000 |
| 基金项目： | 锡林郭勒职业学院重点科研课题(ZD2017-10); 锡林郭勒生物工程研究院重点科研课题(2016002) |
| 摘 要： | 为了研发具有自主知识产权的猪源性成分检测的引物和探针,比对猪、牛、羊、马、鸡、鸭、兔、鹅等8种动物的线粒体基因组,筛选并设计猪特异性的引物和探针组合进行荧光定量PCR。研究结果表明,所研发的猪源性成分检测的引物和探针仅对猪相关DNA模板有典型扩增曲线,对其他动物来源的DNA模板无扩增,具有高度的猪源性特异性。灵敏度实验表明,利用此引物和探针的检测方法具有高度的灵敏度,可达1 pg级。肉制品中猪源性定量检测实验说明此方法具有较好的定量检测能力。此引物和探针组合适用于猪源性食品和农畜产品的检验检测。 |
| 关 键 词： | 猪源性成分 引物 探针 |
| 收稿时间： | 2018-03-21 |
Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR |
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| GUO Liang,LI Chundong,XU Weiliang,GUO Yuansheng.Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR[J].Acta Agriculturae Zhejiangensis,2019,31(1):74. | |
| Authors: | GUO Liang LI Chundong XU Weiliang GUO Yuansheng |
| Institution: | 1. Xilingol Vocational College, Xilinhot 026000,China; 2. Xilingol Institute of Bioengineering, Xilinhot 026000,China; 3.Xilingol Food Testing and Risk Assessment Center, Xilinhot 026000,China |
| Abstract: | The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of TaqMan PCR. The results showed that the technique based on the primers and probe was specific for species identification and quantification, and was able to detect 1 pg template. This biotechnological method based on TaqMan PCR was appropriate to identify porcine-derived materials in farm products and foods. |
| Keywords: | porcine-derived material primer probe |
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