| 葡萄砧木组培快繁研究 |
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| 引用本文： | 刘晓芹,冯美,王振平,平吉成.葡萄砧木组培快繁研究[J].安徽农业科学,2010,38(17):8866-8868,8876. |
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| 作者姓名： | 刘晓芹 冯美 王振平 平吉成 |
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| 作者单位： | 国家葡萄产业技术体质量控制研究室,葡萄与葡萄酒教育部工程研究中心,宁夏大学,宁夏银川,750021;国家葡萄产业技术体质量控制研究室,葡萄与葡萄酒教育部工程研究中心,宁夏大学,宁夏银川,750021;国家葡萄产业技术体质量控制研究室,葡萄与葡萄酒教育部工程研究中心,宁夏大学,宁夏银川,750021;国家葡萄产业技术体质量控制研究室,葡萄与葡萄酒教育部工程研究中心,宁夏大学,宁夏银川,750021 |
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| 摘 要： | 目的]建立葡萄无病毒苗木繁育体系。方法]以3个葡萄砧木品种3309、101-14 mg、trup的单芽茎段为材料,对其进行一定时间的预培养,探讨外植体来源、取材季节、培养基类型、激素配比等关键因素对葡萄组培快繁的影响。结果]预培养后取材的外植体的污染率和褐死率分别为20.00%~28.33%和10.00%~13.33%,室外直接取材的外植体的污染率和褐死率分别为56.67%~81.67%和20.00%~25.00%;以1/2MS为基本培养基最有利于葡萄外植体萌发,各品种腋芽的萌发率均达到60%~72%;春季取材的外植体萌芽率较高,且萌发所需时间较短;3个品种的最适初代培养基为1/2MS＋0.05 mg/L 6-BA＋0.2 mg/L IBA,最适继代和生根培养基为1/2MS＋0.05 mg/L IBA和1/2MS＋0.2 mg/L IBA。结论]该研究对促进葡萄产业化发展具有积极意义。
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| 关 键 词： | 葡萄砧木 组织培养 快繁 |
Study on Tissue Culture and Rapid Propagation of Grape Rootstock |
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| Institution: | LIU Xiao-qin et al(Quality Control Lab of National Grape Industry Technology System,Engineering Research Center of Grape and Grape Wine,Ministry of Education,Ningxia University,Yinchuan,Ningxia 750021) |
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| Abstract: | Objective]The study was to establish the virus-free seedling breeding system of grape.Method] The shoot stem-segments with single bud of 3 grape rootstock varieties 3309,101-14 mgt and rup were taken as the materials.They were pre-cultured for some time to explore the effects of key factors such as explant source,sampling seasons,medium types and hormone ratio on tissue culture and rapid propagation of grape.Results]The contamination rate and frequency of callus brown of the pre-cultured explants were 20.00%~28.33% and10.00%-13.33% resp,and those of explants sampled outside were 56.67%-81.67% and 20.00%-25.00% resp.1/2MS basic medium was the most beneficial to germination of grape explants,and the germination rate of axillary buds of all varieties were 60%-72%.The germination rate of explants sampled in spring was higher and the required time of germination was shorter.The optimum initial medium for 3 varieties was 1/2MS +0.05 mg/L 6-BA+0.2 mg/L IBA,the optimum subculture and rooting medium were 1/2MS +0.05 mg/L IBA and 1/2MS +0.2 mg/L IBA.Conclusion]The study had positive significance in accelerating industrial development of grape. |
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| Keywords: | Grape rootstock Tissue culture Rapid propagation |
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