| 二斑叶螨几丁质酶基因的克隆及特性分析 |
| |
| 引用本文： | 张道伟,钱正敏,张正玲,等.二斑叶螨几丁质酶基因的克隆及特性分析[J].广东农业科学,2015,42(12):128-134. |
| |
| 作者姓名： | 张道伟 钱正敏 张正玲 等 |
| |
| 作者单位： | 遵义师范学院生命科学学院/贵州省赤水河流域动物资源保护与应用研究特色重点实验室,贵州遵义,563002 |
| |
| 基金项目： | 贵州省科学技术基金，遵义师范学院博士基金 |
| |
| 摘 要： | 几丁质酶广泛存在于自然界的许多物种,并参与几丁质降解、蜕皮、免疫和致病性等诸多功能.采用同源克隆结合RACE-PCR的方法,首次从螨类中克隆到二斑叶螨几丁质酶基因(TuChi)的cDNA全长序列(GenBank登录号:JQ041366).TuChi全长1 822 bp,包含3'非翻译区(UTR)为150 bp和5'UTR为52 bp,开放阅读框(ORF)为1 620 bp,编码539个氨基酸.预测的相对分子量为60.7 ku,理论等电点为5.99.二斑叶螨几丁质酶具有几丁质酶典型的结构域,包括1个N-端信号肽序列、1个几丁质催化区域、1个几丁质结合域,以及连接催化区和结合域之间的连接区域.与其他物种几丁质酶进化树比较分析表明,TuChi与昆虫几丁质酶家族Group 1具有较高的同源性,推测其与二斑叶螨的蜕皮密切相关.
|
| 关 键 词： | 二斑叶螨 几丁质酶基因 基因克隆 结构域 序列分析 |
Cloning and characteristics analysis of a chitinase gene from Tetranychus urticae Koch |
| |
| ZHANG Dao-wei,QIAN Zheng-min,ZHANG Zheng-ling,LUO Ying.Cloning and characteristics analysis of a chitinase gene from Tetranychus urticae Koch[J].Guangdong Agricultural Sciences,2015,42(12):128-134. |
| |
| Authors: | ZHANG Dao-wei QIAN Zheng-min ZHANG Zheng-ling LUO Ying |
| |
| Abstract: | Chitinases are widely distributed in nature as they are found in many species with different functions
including chitin digestion, molting, defense/immunity and pathogenicity. In this study, Tetranychus urticae chitinase
gene (TuChi) was first reported from mites using techniques of homological cloning and RACE-PCR. The full length
of cDNA sequence was 1 882 bp (GenBank number: JQ041366), containing a 3UTR (untranslated region) of 150 bp,
a 5UTR of 52 bp. The ORF (Open Reading Frame) of Tuchi encoding cDNA consisted of 1 620 bp with 539 amino
acids residues. Its deduced amino acid composition revealed a calculated molecular weight of 60.7 kua and
theoretical PI of 5.99. TuChi protein was composed of N-terminal leading signal peptide, catalytic domain, linker
region and C-terminal chitin-binding domain, showing a typical insect chitinase domain structure. Phylogenetic
analysis showd that there were high homologies between TuChi and insect chitinase family of Group 1, implicating
that TuChi may have an important role during molting stage of T. urticae. |
| |
| Keywords: | Tetranychus urticae chitinase gene gene cloning domain structure sequence analysis |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
