| 罗非鱼乙酰胆碱酯酶的组织分布尧动力学特性及农药敏感性分析 |
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| 引用本文： | 丁运华,谭笑燕,何玉君,等.罗非鱼乙酰胆碱酯酶的组织分布尧动力学特性及农药敏感性分析[J].广东农业科学,2015,42(12):111-115. |
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| 作者姓名： | 丁运华 谭笑燕 何玉君 等 |
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| 作者单位： | 惠州学院生命科学系,广东惠州,516007 |
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| 基金项目： | 广东省科技计划项目，惠州市科技计划项目 |
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| 摘 要： | 鱼体内乙酰胆碱酯酶(AChE)活性的降低是水体环境农药污染的重要生物化学标记.以奥利亚罗非鱼(Oreochromis aurea)肌肉、肝脏和脑组织为材料,研究乙酰胆碱酯酶的组织分布、动力学性质及12种杀虫剂(9种有机磷类、3种氨基甲酸酯类)对酶的抑制作用.结果表明:脑组织蛋白含量最低(1.53 mg/mL),比活力最高(1.34μmol/min· mg).脑组织AChE的Km值最小(0.160 mmol/L),其次是肌肉AChE (0.227 mmol/L),最高是肝AChE(0.902 mmol/L).脑组织AChE的Vmax最大(2.35 μmol/min ·mgp),其次是肝组织,最小是肌肉组织(0.63μmol/min· mgp).12种农药对肌肉AChE抑制作用强弱顺序为:敌敌畏＞辛硫磷＞敌百虫＞呋喃丹＞乐果＞甲基异柳磷＞马拉硫磷＞氧化乐果＞丁硫克百威＞甲胺磷＞对硫磷＞灭多威,对脑AChE抑制作用强弱顺序为:敌敌畏＞呋喃丹＞灭多威＞敌百虫＞辛硫磷＞氧化乐果＞乐果＞对硫磷＞丁硫克百威＞甲基异柳磷＞马拉硫磷＞甲胺磷,对肝脏AChE抑制作用强弱顺序为:呋喃丹＞敌敌畏＞辛硫磷＞甲基异柳磷＞灭多威＞敌百虫＞氧化乐果＞丁硫克百威＞对硫磷＞马拉硫磷＞甲胺磷＞乐果.
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| 关 键 词： | 乙酰胆碱酯酶 组织分布 IC50 动力学性质 罗非鱼 |
Tissue distribution, kinetic characters and sensitivity to insecticides of acetylcholinesterase in Oreochromis aurea |
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| DING Yun-hua,TAN Xiao-yan,HE Yu-jun,LI Su-ling.Tissue distribution, kinetic characters and sensitivity to insecticides of acetylcholinesterase in Oreochromis aurea[J].Guangdong Agricultural Sciences,2015,42(12):111-115. |
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| Authors: | DING Yun-hua TAN Xiao-yan HE Yu-jun LI Su-ling |
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| Abstract: | Acetylcholinesterase (AChE, EC3.1.1.7) plays an important role in environmental insecticide detection. Using muscle, liver and brain tissue as materials, tissue distribution and kinetic characters of acetylcholinesterase in Oreochromis aurea and its sensitivity to 12 kinds of insecticides (including 9 organophosphates,3 carbamates) were studied. The results showed that, brain tissue had the highest specific activity (1.34 mol/minmg) while the lowest protein content. Km of AChE in brain tissue was the minimal with 0.160 mmol/L, Km of AChE in muscle was second with 0.227 mmol/L, and Km of AChE in liver was the highest with 0.902 mmol/L. Vmax of AChE in brain tissue was the highest with 2.35 mol/minmgp, followed by liver tissue, and then muscle tissue with 0.63mol/minmgp. Inhibition test showed that 12 insecticides exhibited strong inhibitions on AChE with the order inmuscle tissue as dichlorvos > phoxim > dipterex > furadan > rogor > isofenphosmethyl > malathion > omethoate>carbosulfan > methamidophos > parathion > methomyl, the order in brain tissue as dichlorvos > furadan > methomyl> dipterex > phoxim > omethoate > rogor > parathion > carbosulfan > isofenphosmethyl > malathion > methamidophos, the order in liver tissue as furadan > dichlorvos > phoxim > isofenphosmethyl > methomyl > dipterex> omethoate> carbosulfan > parathion > malathion >methamidophos>rogor |
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| Keywords: | acetylcholinesterase tissue distribution IC50 kinetic characters Oreochromis aurea |
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