| 猪繁殖与呼吸综合征病毒nsp2基因缺失株RT-PCR检测方法的建立 |
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| 引用本文： | 李玉峰,王先炜,陈闻,姜平,许家荣.猪繁殖与呼吸综合征病毒nsp2基因缺失株RT-PCR检测方法的建立[J].南京农业大学学报,2009,32(3). |
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| 作者姓名： | 李玉峰 王先炜 陈闻 姜平 许家荣 |
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| 作者单位： | 南京农业大学动物医学院,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金项目，南京农业大学青年科技创新基金 |
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| 摘 要： | 为了快速检测猪繁殖与呼吸综合征病毒(PRRSV) nsp2基因缺失流行株,在对不同PRRSV分离株nsp2基因核苷酸序列分析的基础上,设计了针对nsp2基因的1对兼并PCR引物和1条基因特异性反转录引物.RT-PCR试验结果表明,经典PRRSV S1毒株和高致病性nsp2缺失株SY0608毒株的PCR扩增片段的大小分别为768 bp和678 bp.特异性试验和敏感性试验证明,该检测方法特异性较好,但敏感性相对较低,可用于快速鉴别诊断PRRSV nsp2基因缺失毒株和经典PRRSV毒株.
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| 关 键 词： | nsp2基因 基因缺失 |
Establishment of RT-PCR for detection of nsp2 deletion porcine reproductive and respiratory syndrome virus |
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| LI Yu-feng,WANG Xian-wei,CHEN Wen,JIANG Ping,XU Jia-rong.Establishment of RT-PCR for detection of nsp2 deletion porcine reproductive and respiratory syndrome virus[J].Journal of Nanjing Agricultural University,2009,32(3). |
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| Authors: | LI Yu-feng WANG Xian-wei CHEN Wen JIANG Ping XU Jia-rong |
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| Institution: | College of Veterinary Medicine;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | In order to rapidly detect nsp2 gene deleted isolates,based on nsp2 gene sequences of different isolates accessed in GenBank,a pair of degenerate PCR primers and one gene specific reverse primer were designed.RT-PCR results showed that the PCR products for nsp2 deleted PRRSV and conventional isolates were 768 bp and 678 bp in length,respectively.Sensitivity and specificity tests results showed this method had good sensitivity and specificity.It could be used to rapidly differentiate nsp2 deleted isolates an... |
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| Keywords: | PRRSV RT-PCR |
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