| 2株奶牛乳房炎分离菌的耐药性检测及中草药体外抑菌试验 |
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| 引用本文： | 张为民,邢福珊,徐永平,骆曜华,马云,赵春平,李勤凡,逄国梁.2株奶牛乳房炎分离菌的耐药性检测及中草药体外抑菌试验[J].西北农业学报,2009,18(5):7-11. |
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| 作者姓名： | 张为民 邢福珊 徐永平 骆曜华 马云 赵春平 李勤凡 逄国梁 |
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| 作者单位： | 1. 西北农林科技大学,动物医学学院,陕西杨凌,712100
2. 陕西省畜牧技术推广总站,陕西西安,710016 |
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| 基金项目： | 陕西省农业厅推广项目 |
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| 摘 要： | 对陕西省某奶牛场的临床型乳房炎病牛奶样中分离的金黄色葡萄球菌和表皮葡萄球菌进行了14种常用抗生素的耐药性筛选和中草药体外抑菌研究.2株致病菌对青霉素类药物(除氧哌嗪青霉素外)、复达欣和磺胺类药物有耐药性,而对庆大霉素和万古霉素等敏感.所选的中草药石榴皮和连翘对金黄色葡萄球菌的效果最好,最低杀菌浓度(MBC)均为31.25 mg/mL,穿心莲次之为62.50 mg/mL;对表皮葡萄球菌抑菌效果,石榴皮MBC为7.81 mg/mL,大黄为31.25 mg/mL,穿心莲、金银花和连翘为125.00 mg/mL,白附子和紫花地丁为62.50 mg/mL.自拟复方对金黄色葡萄球菌的抑菌环大小为18.58 mm,MBC为62.50 mg/mL;对表皮葡萄球菌的抑菌环大小为23.82 mm,MBC为31.25 mg/mL.筛选的中草药复方可以作为治疗乳房炎的药物.
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| 关 键 词： | 乳房炎 葡萄球菌 药敏试验 中草药 |
| 收稿时间： | 2009/3/9 0:00:00 |
| 修稿时间： | 2009/5/8 0:00:00 |
The Research on the Resistance and the Bacteriostasis of Chinese Herbal Medicine in Vitro about Two Bacteria Isolated from Mastitis in Dairy Cows |
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| ZHANG Weimin,XING Fushan,XU Yongping,LUO Yaohu,MA Yun,ZHAO Chunping,LI Qinfan and PANG Guoliang.The Research on the Resistance and the Bacteriostasis of Chinese Herbal Medicine in Vitro about Two Bacteria Isolated from Mastitis in Dairy Cows[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(5):7-11. |
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| Authors: | ZHANG Weimin XING Fushan XU Yongping LUO Yaohu MA Yun ZHAO Chunping LI Qinfan and PANG Guoliang |
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| Institution: | College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Animal medicine, Northwest A&F University, Yangling Shaanxi 712100, China;Animal Husbandry Technical Extension General Station of Shaanxi Province, Xi''an Shaanxi 710016 China |
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| Abstract: | The S.aureus and S.epidermidis separated from Clinical mastitis milk on one cattle farm in Shaanxi Province were carried through 14 kinds of commonly used antibiotics resistance filter and Chinese herbal.Two pathogenic bacteria to Penicillin-type drugs(except Piperacillin),Fortum and TMP/SMZ are resistant,wherea Gentamicin,Vancomycin and so on are sensitive.Purcica grarcatum and Forsythiasuspercsa are best to S.aureusin effect,MBC are 31.25 mg/mL,followed by Arcdrographis parciculata to 62.50 mg/mL.For the bacterio static effect on S.epi dermidis,the Purcica grarcatum MBC is 7.81 mg/mL,the Rheum officircale MBC is 31.25 mg/mL,the MBC of Andrcdrographis parciculata,Lorcicera japonica as well as Forsythia suspercsa are 125.00 mg/mL,and the MBC of Typhorcium gigarcteum and Viola yefoercsis are 62.50 mg/mL.Compound to inhibitory zone size of S.aureus is 18.58 mm,and it''s MBC is 62.50 mg/mL.The inhibitory zone size of S.epidermidis is 23.82 mm,and it''s MBC is 31.25 mg/mL.The filtered Chinese herbal medicine compound can be used for the treatment of mastitis drugs. |
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| Keywords: | Mastitis Staphylococci Medicine sensitivity test Chinese herbal medicine |
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