Genetic diversity of diploid Japanese strawberry species based on microsatellite markers |
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| Authors: | Wambui Njuguna Kim E Hummer Christopher M Richards Thomas M Davis Nahla V Bassil |
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| Institution: | (1) Department of Horticulture, 4017 Agriculture and Life Science Building, Oregon State University, Corvallis, Oregon 97331, USA;(2) United States Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Rd., Corvallis, Oregon 97333, USA;(3) United States Department of Agriculture, Agricultural Research Service, National Center for Genetic Resources Preservation, 111 South Mason Street, Fort Collins, Colorado 80521, USA;(4) Department of Biological Sciences, Rudman Hall, University of New Hampshire, Durham, New Hampshire 03824, USA |
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| Abstract: | The United States Department of Agriculture (USDA)—Agricultural Research Service (ARS)—National Clonal Germplasm Repository
(NCGR) in Corvallis, Oregon, is a genebank that preserves strawberry genetic resources. Representatives of two Japanese diploid
species, Fragaria iinumae Makino and F. nipponica Makino were collected for conservation by the NCGR during an expedition to Hokkaido, Japan. Fragaria iinumae may be a genome contributor to the cultivated octoploid strawberries. The objective of this study was to evaluate the genetic
diversity of these two species by using simple sequence repeat (SSR) markers. Twenty of 82 Fragaria-derived SSRs, polymorphic among and within the two species, were selected for genetic analysis of 137 accessions. Genetic
diversity, based on the proportion of shared alleles between the two species, in F. nipponica (0.4542) and F. iinumae (0.1808) was significantly different. Three wild interspecific hybrids were identified from intermediate memberships in the
two diploid species groups revealed by using the clustering program, Structure. Principal coordinate analysis followed by
non-parametric modal clustering (PCO-MC) grouped accessions into two clusters representing the two diploid species. Further
clustering within the species groups generated with the program, STRUCTURAMATM, resulted in seven subclusters in F. iinumae and three in F. nipponica, which may represent breeding populations appropriate for clonal conservation. Long-term preservation of the species populations
and the limited number of hybrids on the island is discussed relative to their geographical distribution and the geological
history of Hokkaido Island. |
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