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桦褐孔菌液体深层发酵条件优化研究
引用本文:申进文,李燕,王振河,霍云凤,王斌.桦褐孔菌液体深层发酵条件优化研究[J].河南农业科学,2007(8):88-92.
作者姓名:申进文  李燕  王振河  霍云凤  王斌
作者单位:1. 河南农业大学,生命科学学院,河南,郑州,450002
2. 河南科技学院,河南,新乡,453003
摘    要:以生物量为指标,研究了对桦褐孔菌液体深层发酵影响较大的主要因素,即温度、发酵时间、初始pH、接种量、摇床转速,并运用中心旋转组合设计法对发酵条件进行了优化,确定了其最佳范围。结果表明,5个因子对生物量的影响由大到小依次为初始pH、发酵时间、接种量和摇床转速、温度,而当温度、发酵时间、初试pH、接种量和摇床转速分别为28.6℃,125 h,5.9,10.5%和135r/min时,获得的生物量最大,为22.91 g/L。

关 键 词:桦褐孔菌  发酵条件  生物量  中心旋转组合设计
文章编号:1004-3268(2007)08-0088-05
修稿时间:2007-05-09

Optimization of the Fermenting Conditions for Phaeoporus obliquus Liquor in Submerged Fermentation
SHEN Jin-wen,LI Yan,WANG Zhen-he,HUO Yun-feng,WANG Bin.Optimization of the Fermenting Conditions for Phaeoporus obliquus Liquor in Submerged Fermentation[J].Journal of Henan Agricultural Sciences,2007(8):88-92.
Authors:SHEN Jin-wen  LI Yan  WANG Zhen-he  HUO Yun-feng  WANG Bin
Institution:1. College of Life Science of Henan Agricultural University, Zhengzhou 450002, China 2. Henan Institute of Science and Technology, Xinxiang 453003 ,China
Abstract:Different factors,including temperature,fermentation time,initial pH,inoculum and rotary speed were studied comparatively using biomass as a director during the submerged fermentation of Phaeoporus obliquus liquor,and were optimized using central composite rotatable design(CCRD).The results indicated that the five factors have different effects on biomass,with a order from high to low: initial pH,fermentation time,inoculum and rotary speed,temperature.The maximum biomass(22.91 g/L) was acquired under the conditions of temperature 28.6℃,fermentation time 125 h,initial pH5.9,inoculum 10.5%(v/v)and rotary speed 135 r/min.
Keywords:P  obliquus  Fermentation condition  Biomass  Central composite rotatable design(CCRD)
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