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Positive effects of treatment of donor cells with aphidicolin on the preimplantation development of somatic cell nuclear transfer embryos in Chinese Bama mini-pig (Sus Scrofa) |
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| Authors: | Ting-Yu ZHANG Jian-Jun DAI Cai-Feng WU Xiao-Long GU Liang LIU Zhi-Qiang WU Yi- Ni XIE Bin WU Hui-Lan CHEN Yao LI Xue-Jin CHEN De-Fu ZHANG |
| Institution: | 1. Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences
Shanghai Municipal Key Laboratory of Agri-genetics and Breeding;2. Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences Shanghai Municipal Key Laboratory of Agri-genetics and Breeding Animal Science and Technology College, Sichuan Agricultural University, Ya'an, China;3. Department of Laboratory Animal Science, School of Medicine, Shanghai Jiao Tong University, Shanghai |
| Abstract: | To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types and aphidicolin (APC) treatment on in vitro development of reconstructed embryos. Oviduct epithelial cells (OEC), ear fibroblast cells (EFC) and cumulus cells (CC) derived from mini-pigs were treated with serum starvation only or serum starvation followed by treatment of 0.1 µg/mL APC. The reconstructed embryos were cultured for 7 days to evaluate their developmental competency. Cleavage and blastocyst formation rates of reconstructed embryos derived from the OEC by APC treatment were significantly higher than the serum starvation (61.82% vs. 56.25%, 24.55% vs. 17.86%; P < 0.05). The cleavage rate from the EFC was significantly increased by APC treatment compared to serum starvation only (63.36% vs. 57.01%; P < 0.05). In the ooctyes with the CC, the reconstructed embryos could yield high blastocyst formation rate by APC treatment (29.63%; P < 0.05). In the presence of APC, CC gave rise to the highest cleavage and blastocyst formation rates among the three cell types. Therefore, our results suggest that treatment of CC with serum starvation plus APC prior to nuclear transfer is more suitable in SCNT of mini-pigs. |
| Keywords: | aphidicolin cell type mini-pig somatic cell nuclear transfer |