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| 香石竹枯萎病及其病原菌基于rDNA的遗传分析 | |
| 引用本文： | 陈晓波,张云峰,官会林,周滔,毕云青,杨泮川.香石竹枯萎病及其病原菌基于rDNA的遗传分析[J].中国农学通报,2010,26(11):40-44. |
| 作者姓名： | 陈晓波 张云峰 官会林 周滔 毕云青 杨泮川 |
| 作者单位： | 1. 云南师范大学能源与环境学院,昆明,650092;云南师范大学生命科学学院,昆明,650092 2. 云南师范大学生命科学学院,昆明,650092 3. 云南师范大学能源与环境学院,昆明,650092 4. 云南省农科院农业环境资源研究所,昆明,650205 5. 云南英茂花卉公司,昆明,650228 |
| 基金项目： | 国家自然科学基金，国家科技支撑项目 |
| 摘 要： | 随着栽培年限的延长,昆明地区香石竹栽培中的枯萎病害发生日愈严重。通过田间调查及病原菌分离、鉴定、田间接种确定香石竹枯萎病的病原菌为立枯丝核菌。并以真菌通用ITS引物对所分离的3株病原菌进行PCR扩增,结果表明扩增片段为750bp,测序后去除引物序列长度为505bp,所分离3株立枯丝核菌的序列完全一致,该序列与马铃薯、西红柿、水稻、棉花立枯丝核菌的同源性在85%以上,表明所分离于香石竹枯萎病的3个病原菌为立枯丝核菌。利用DNAMAN4.0进行系统进化树分析,表明分离菌株与大麦、四季豆、马铃薯、西红柿、水稻、棉花等的立枯丝核菌归为一类,而落叶松、柠檬、青椒、剑兰、苹果、咖啡等的立枯丝核菌为另一类。在前茬作物交配类型无法确定的情况下,前茬作物的致病菌不仅存在交叉感染的可能,同时还可能产生新的变异类型。因此,在香石竹栽培过程中,应尽量避免在前茬作物为遗传学上相对较近的作物栽培土壤中种植。 |
| 关 键 词： | 农业电子商务 农业电子商务 发展模式 配对率 点击率 |
| 收稿时间： | 2010-01-07 |
| 修稿时间： | 2010-01-31 |
rDNA Genetic Analysis of Rhizoctonia Solani CausingDamping-off of Carnation |
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| Chen Xiaobo,Zhang Yunfeng,Guan Huilin,Zhou Tao,Bi Yunqing,Yang Banch.rDNA Genetic Analysis of Rhizoctonia Solani CausingDamping-off of Carnation[J].Chinese Agricultural Science Bulletin,2010,26(11):40-44. | |
| Authors: | Chen Xiaobo Zhang Yunfeng Guan Huilin Zhou Tao Bi Yunqing Yang Banch |
| Institution: | Chen Xiaobo, Zhang Yunfeng, Guan Huilin, Zhou Tao, Bi Yunqing, Yang Banchuan (The School of Energy and Environmental Sciences, Yunnan Normal University, Kunming 650092; 2The School of Life Sciences, Yunnan Normal University, Kunming 650092; 3Yunnan Institute of Environmental Resources, Agriculture Academy, Kunming 650205; 4Yingmao Flower Company of Yunnan, Kunming 650228) |
| Abstract: | This article determine pathogen in carnation which grows in Kunming is Rhizoctonia solani, after field investigation and sampling, indoor isolation, identification, separation of strain field and rDNA appraisal in separate strains. And compare with homology sequence, the results showed that the homology of different host regulatory bacteria homology, homology of minimum and maximum 98%, for 52%. From the homology analysis and the comprehensive analysis of the evolutionary tree, Rhizoctonia solani as a wider range of host pathogenic bacteria, its genome in the evolutionary process of mutation, relative mutual infection. Therefore, in the process of cultivation carnation, we should avoid planting crops in which genetics is relatively close before crop. |
| Keywords: | Carnation Rhizoctonia solani Genetic specificityzz |
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