Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe |
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| Authors: | Frederic Aparicio Salvador Soler José Aramburu Luis Galipienso Fernando Nuez Vicente Pallás Carmelo López |
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| Institution: | (1) Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia (UPV-CSIC), Avenida de los Naranjos s/n, 46022 Valencia, Spain;(2) Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universidad Politécnica de Valencia (COMAV-UPV), Avenida de los Naranjos, s/n, 46022 Valencia, Spain;(3) Institut de Recerca i Tecnología Agroalimentaries (IRTA), Ctra. de Cabrils s/n Cabrils, 08348 Barcelona, Spain |
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| Abstract: | A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the
following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition,
we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato
plants with the polyprobe.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Diagnosis Dig-RNA polyprobe Tissue-printing Tomato |
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