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Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
Institution:1. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce), CONICET – INTA, Ruta 226 Km 73.5, (7620), Balcarce, Buenos Aires, Argentina;2. Instituto de Análisis Fares Taie, Rivadavia 3331, (7600), Mar del Plata, Buenos Aires, Argentina;3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina;4. Centro de Investigación en Abejas Sociales, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, UNMDP, Funes, 3350, (7600), Mar del Plata, Buenos Aires, Argentina;5. Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5, (7620), Balcarce, Buenos Aires, Argentina;6. Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226 Km 73.5, (7620), Balcarce, Buenos Aires, Argentina;1. Department of Animal Biology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;2. Department of Genetics and Evolution, Microbiology and Immunology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;3. Superintendência de Controle de Endemias, Seção Técnica de Pesquisa, Controle deVetores, Campinas, São Paulo, Brazil;4. Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil;1. Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;3. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;1. Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil;2. Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Rio de Janeiro, Brazil;1. Laboratory of Veterinary Microbiology And Immunology, Federal University of Acre, Dias Martins St., 2374-2544 Rio Branco, Acre, Brazil;2. Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Prof. Hernani Mello St., 101, São Domingos, 24210-130 Niterói, Rio de Janeiro, Brazil;3. Estacio de Sa University, Boca do Mato St., 850, 22783-320, Vargem Pequena, Rio de Janeiro, Brazil;4. Laboratory of Epidemiology and Molecular Sistematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Leônidas Deane Pavilion, Avenue Brasil, 4365, Manguinhos 21040-900, Rio de Janeiro, Brazil
Abstract:Leptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.
Keywords:Bovine abortion  Fetuses  Diagnosis  qPCR
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