Brucella melitensis infection in dog: a critical issue in the control of brucellosis in ruminant farms |
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| Institution: | 1. College of Animal Science, Tarim University, Alar, Xinjiang 843300, China;2. Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China;1. College of Veterinary Medicine, Midwestern University, Glendale, AZ, United States;2. Department of Animal Care and Technologies, Arizona State University, United States;3. Department of Microbiology and Immunology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, United States;4. Department of Pathobiological Sciences, University of Wisconsin-Madison School of Veterinary Medicine, United States;5. Department of Biomedical Sciences, Idaho College of Osteopathic Medicine, United States;1. Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India;2. Goa State Co-Operative Milk Producers’ Union Limited, Curti, Ponda 403 401, India;3. Network Project on Brucellosis, Department of Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India;1. School of Medical Technology, Baotou Medical College, Baotou 014040 China;2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping, Beijing 102206, China;3. Inner Mongolia Autonomous Region Center for Comprehensive Disease Control and Prevention, Huhhot 010031, China;4. Sanya People’ s Hospital, Sanya 572000, Hainan, China;5. Ulanqab Centre for Endemic Disease Prevention and Control, Jining, 012000, Inner Mongolia, China;6. Qinghai Institute for Endemic Diseases Prevention and Control, Xining, 811602, China;7. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China |
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| Abstract: | Canine brucellosis is a contagious disease associated with health implications for humans as well as for a wide range of wild and domesticated animals. In this study, 173 dog blood specimens were sampled from herding dogs in three different provinces including Tehran (n = 127), Qom (n = 40) and Alborz (n = 6) provinces. The presence of Brucella antibodies was determined using Rose Bengal plate test (RBPT), slow agglutination test (SAT) and 2-mercaptoethanol (2-ME), respectively. The seropositive samples were further screened using blood culture and PCR tests to identify and differentiate the implicated Brucella species. According to our results, 24.3% (42/173), 13.8% (24/173) and 6.3% (11/173) of blood samples were tested positive using RBPT, SAT and 2-ME, respectively. However, among 42 seropositive samples, only 38.1% (16/42) and 14.2% (6/42) were positive by PCR and culture, respectively. Brucella melitensis biovar 1 and biovar 2 was isolated from the bacterial cultures of 6 blood samples and confirmed by biotyping, AMOS PCR and Bruce-ladder PCR assays. These findings highlight the potential risk of Brucella transmission from dog to humans along with other livestock and reflect the critical role of infected dogs in the persistence of Brucella infections among ruminant farms. This study stresses the need for further epidemiological investigations on canine brucellosis among herding dogs and suggests the systematic screening of the disease among companion animals such as dogs in order to improve brucellosis surveillance and control programs. |
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| Keywords: | canine brucellosis serology test molecular test |
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