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| 葡聚六糖对烟草悬浮培养细胞活性氧的作用 | |
| 引用本文： | 孙艳秋,李宝聚,石延霞,陈捷.葡聚六糖对烟草悬浮培养细胞活性氧的作用[J].吉林农业大学学报,2009,31(1). |
| 作者姓名： | 孙艳秋 李宝聚 石延霞 陈捷 |
| 作者单位： | 1. 沈阳农业大学植物保护学院,沈阳,110161 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 上海交通大学农业与生物学院,上海,201101 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金重点项目 |
| 摘 要： | 研究了葡聚六糖对烟草悬浮培养细胞活性氧的作用.结果表明:葡聚六糖可以诱导烟草悬浮细胞活性氧的进发,活性氧进发蜂值出现在30 min,同时活性氧相关酶系活性也升高,超氧化物歧化酶(SOD)活性最高峰在处理后60 min,过氧化物酶(POD)和过氧化氢酶(CAT)变化趋势与活性氧相似.添加不同抑制剂EGTA、新霉素、氯化锂(LiCl)等测定发现,细胞胞内和胞外Ca2+参与调控葡聚六糖诱导的活性氧进发. |
| 关 键 词： | 葡聚六糖 烟草悬浮细胞 活性氧 |
Effect of Active Oxygen in Tobacco Suspension Cultures Induced by Glucohexaose |
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| SUN Yan-qiu,LI Bao-ju,SHI Yan-xia,CHEN Jie.Effect of Active Oxygen in Tobacco Suspension Cultures Induced by Glucohexaose[J].Journal of Jilin Agricultural University,2009,31(1). | |
| Authors: | SUN Yan-qiu LI Bao-ju SHI Yan-xia CHEN Jie |
| Institution: | 1.College of Plant Protection;Shenyang Agricultural University;Shenyang 110161;China;2.Institute of Vegetables and Flowers;CAAS;Beijing 100081;3.College of Agriculture and Biology;Shanghai Jiaotong University;Shanghai 201101;China |
| Abstract: | Active oxygen could be induced to burst by glucohexaose in tobacco suspension cultures,and oxidative burst maximum was in 30 minutes,and relative enzyme activity of active oxygen was also improved in cells.The maximum of SOD activities was in 60 minutes,and the current of POD and CAT activities changes were similar to active oxygen.By adding different inhibitors such as EGTA,neomycin,LiCl and so on,intracellar and extracellar Ca2+ were indicated to be able to take part in regulating active oxygen burst indu... |
| Keywords: | glucohexaose tobacco suspension culture active oxygen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |