| 盐提法提取波纹巴非蛤全脏器中糖蛋白的工艺条件研究 |
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| 引用本文： | 周国杰,黄晓玲,范秀萍.盐提法提取波纹巴非蛤全脏器中糖蛋白的工艺条件研究[J].现代农业科技,2009(21):260-261. |
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| 作者姓名： | 周国杰 黄晓玲 范秀萍 |
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| 作者单位： | 广东海洋大学食品科技学院,广东湛江,524006 |
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| 摘 要： | 对波纹巴非蛤全脏器中的糖蛋白的分离提取条件进行研究，结果表明：以0．5moL／L的NaCl溶液为浸提液，料液比为1：12，75℃水浴浸提90min，提取液经透析浓缩，再以60％乙醇终浓度沉淀，得到糖蛋白粗品，其得率为1．64％，总糖与可溶性蛋白含量分别为38．3％和17．1％。
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| 关 键 词： | 波纹巴非蛤 糖蛋白 分离提取 工艺条件 |
The Process Conditions for Glycoprotein Extraction from Paphia Undulata |
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| Authors: | ZHOU Guo-jie HUANG Xiao-ling FAN Xiu-ping |
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| Institution: | (College of Food Science and Technology, Guangdong Ocean University, Zhanjiang Guangdong 524006 ) |
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| Abstract: | Experiments were carried out to isolate the glycoprotein from the whole viscera of the Paphia undulata.The results showed that the optimal separated condition was: the material was immersed in 0.5moL/L NaCl solution,the ratio of material to NaCl solution was 1 ∶12,being extracted with 75℃ water-bath for 90min,then the solution was dialyzed with water,concentrated and precipitated with alcohol to 60%,the ultimate yield of the coarse glycoprotein was 1.64%.The content of total sugar was 38.3%,while protein was 17.1%. |
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| Keywords: | Paphia undulata glycoprotein isolation and extraction process conditions |
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