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Identification and characterization of <Emphasis Type="Italic">Diaporthe vaccinii</Emphasis> Shear causing upright dieback and viscid rot of cranberry in Poland
Authors:Monika Michalecka  Hanna Bryk  Paulina Seliga
Institution:1.Department of Plant Pathology,Research Institute of Horticulture,Skierniewice,Poland
Abstract:During the summers of 2013–2014, symptoms similar to viscid rot and upright disease were observed on cranberries (Vaccinium macrocarpon) on one plantation in central Poland. The associated fungi were isolated from symptomatic plant tissue. On the basis of morphological and cultural characteristics and the ability of isolated fungi to elicit viscid rot symptoms on cranberry fruits, they were classified as the genus Diaporthe. Further analysis of ITS sequence data allowed for the classification of the newly obtained isolates as D. vaccinii. Additional analysis of genetic diversity using five RAPD and eight ISSR primers constituted additional confirmation of genetic distance existing between closely related D. vaccinii and D. eres species, enabling their differentiation.
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