Genetic variability within <Emphasis Type="Italic">Septoria carvi</Emphasis> Syd.- a pathogen of caraway <Emphasis Type="Italic">Carum carvi</Emphasis> L |
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| Authors: | Ewa Dorota Zalewska Ewa Dorota Król Barbara Anna Abramczyk Agnieszka Furmańczyk Sylwia Okoń |
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| Institution: | 1.Department of Plant Protection,University of Life Sciences in Lublin,Lublin,Poland;2.Department of Agricultural Microbiology,Institute of Soil Science and Plant Cultivation – State Research Institute,Pu?awy,Poland;3.Institute of Plant Genetics, Breeding and Biotechnology,Lublin,Poland |
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| Abstract: | Genetic variability within Septoria carvi isolates obtained from various organs of caraway cultivated in south-eastern and central Poland was studied using the RAPD-PCR technique. The tests were performed using randomly selected primers. The DNA profiles obtained using four primers proved useful in determining genetic variability among the genotypes of Septoria carvi isolates. The present study characterized the differences in the nucleotide sequence within the internal transcribed spacer region of rDNA (ITS1, 5.8S, ITS2) of selected S. carvi isolates and reference strains of Septoria spp. Moreover, eight isolates were sequenced for three loci: actin, calmodulin and translation elongation factor 1-alpha, and the obtained sequences were compared with the sequences of Septoria reference strains affecting other plants of the family Apiaceae. Phylogenetic analysis showed distinct differences of the tested isolates, which allowed to treat them Septoria carvi species affecting the above-ground organs of caraway Carum carvi L. This study is the first report on the genetic characteristics of the species S. carvi. |
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