Protoplasts in the analysis of early plant-pathogen interactions: current applications and perspectives |
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| Authors: | Tim Xing Xiu-Qing Li Andre Laroche Lining Tian Kipkios Tubei Xiaojing Wang |
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| Institution: | 1.Department of Biology and Institute of Biochemistry,Carleton University,Ottawa,Canada;2.Fredericton Research and Development Centre, Agriculture and Agri-Food Canada,Fredericton,Canada;3.Lethbridge Research and Development Center,Agriculture and Agri-Food Canada,Lethbridge,Canada;4.London Research and Development Centre,Agriculture and Agri-Food Canada,London,Canada;5.College of Life Sciences, Guangdong Key Lab of Biotechnology for Plant Development,South China Normal University,Guangzhou,People’s Republic of China |
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| Abstract: | Transient gene expression in protoplasts has been applied to the analysis of gene expression, promoter activity, subcellular localization of proteins, and genome editing. Its advantages have been realized in recent years in various physiological, biochemical, genetic, and molecular biological studies. The omics and systems biology studies of plant development and their interactions with the environment have revitalized this approach. Early plant-pathogen interaction represents one of the most unique signalling research areas. Here, we try to discuss some developments and current applications of the protoplast system. It is for sure that the system will significantly bridge the gap between high throughput approaches and functional analysis in the study of plant-pathogen interactions in the omics era. |
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