| 锌对团头鲂幼鱼血清1H NMR代谢组的影响 |
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| 引用本文： | 蒋明,杨长庚,王卫民,文华,吴凡,刘伟,田娟,陆星,喻丽娟.锌对团头鲂幼鱼血清1H NMR代谢组的影响[J].水产学报,2018,42(6):838-845. |
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| 作者姓名： | 蒋明 杨长庚 王卫民 文华 吴凡 刘伟 田娟 陆星 喻丽娟 |
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| 作者单位： | 中国水产科学研究院长江水产研究所鱼类营养与饲料研究室;华中农业大学水产学院 |
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| 基金项目： | 国家大宗淡水鱼类产业技术体系专项（CARS-45-8）；中央级公益性科研院所基本科研业务费专项（2013JBFM22） |
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| 摘 要： | 为了发掘团头鲂对锌(Zn)敏感的代谢标志物,以3种Zn含量分别为7.4、32.1和332.4 mg/kg的半纯化饲料(分别标记为L、M和H组),喂养初始体质量为(3.6±0.1)g团头鲂12周,应用1H(氢谱)核磁共振波谱法(1H NMR)检测团头鲂血清代谢物的种类和浓度,再用最小二乘法判别(PLS-DA)和变量重要性(VIP)分析3个处理团头鲂血清的代谢轮廓和主要差异代谢物。结果显示,血清中52种代谢物被定性和定量,其中氨基酸及其衍生物23种、有机酸16种、糖类4种、核酸组分4种、维生素3种和其他组分2种,经PLS-DA和VIP分析,3组的代谢轮廓相异,其中L组血清中脯氨酸、丙氨酸、赖氨酸等8种氨基酸和葡萄糖含量较M组高,H组血清中则有12种氨基酸和葡萄糖含量较M组高。差异代谢物依次为脯氨酸、乳酸、葡萄糖、丙氨酸、赖氨酸、谷氨酰胺、肌酸、反式-4-羟基-L-脯氨酸、牛磺酸、丝氨酸、τ-甲基组氨酸、精氨酸、缬氨酸、蛋氨酸和甘露糖。以上结果显示,饲料中Zn缺乏和过量会抑制团头鲂的氨基酸代谢和糖代谢,脯氨酸、乳酸和葡萄糖是团头鲂对Zn敏感的潜在生物标志物。
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| 关 键 词： | 团头鲂 锌 代谢组 脯氨酸 乳酸 |
| 收稿时间： | 2017/4/14 0:00:00 |
| 修稿时间： | 2017/10/10 0:00:00 |
1H NMR-based metabolomics studies on the effect of Zn in blunt snout bream (Megalobrama amblycephala) |
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| JIANG Ming,YANG Changgeng,WANG Weimin,WEN Hu,WU Fan,LIU Wei,TIAN Juan,LU Xing and YU Lijuan.1H NMR-based metabolomics studies on the effect of Zn in blunt snout bream (Megalobrama amblycephala)[J].Journal of Fisheries of China,2018,42(6):838-845. |
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| Authors: | JIANG Ming YANG Changgeng WANG Weimin WEN Hu WU Fan LIU Wei TIAN Juan LU Xing and YU Lijuan |
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| Institution: | Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China;College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China,College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China,Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China and Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, China Academy of Fishery Sciences, Wuhan 430223, China |
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| Abstract: | |
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| Keywords: | Megalobrama amblycephala zinc metabolomics proline lactate |
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