| 养殖鳖的引入对稻田土壤细菌群落结构的影响 |
| |
| 引用本文： | 罗衡,赵良杰,李丰,郭海松,沈竑,刘其根.养殖鳖的引入对稻田土壤细菌群落结构的影响[J].水产学报,2018,42(5):720-732. |
| |
| 作者姓名： | 罗衡 赵良杰 李丰 郭海松 沈竑 刘其根 |
| |
| 作者单位： | 上海海洋大学农业部水产种质资源与利用重点开放实验室;信阳农林学院河南省渔业生物工程技术研究中心;上海市沐雨生态农庄 |
| |
| 基金项目： | 公益性行业（农业）专项（201203083）；上海市高校知识服务平台项目（ZF1206） |
| |
| 摘 要： | 农业—水产复合生产体系(integrated agri-aquaculture system,IAAS)因其具有良好的生态环境效应及能生产满足市场对食品安全需求的绿色稻米而成为了农业可持续发展的主要方向之一。了解这种农水复合系统中土壤细菌群落结构特征对于种养工艺的改进和生态调控的实现具有重要意义。实验采用高通量测序技术比较稻鳖共作(R-T)和稻田单作(R-M)2种模式下稻田表层和根系土壤微生物的群落结构,旨在为稻鳖种养生态学积累资料和为稻鳖综合种养的生产技术优化提供理论依据。高通量测序结果显示,两种模式下稻田表层及根系土壤中主要细菌门类为变形菌门、酸杆菌门、拟杆菌门、硝化螺旋菌门和绿弯菌门。与R-M相比,R-T表层土壤中绿弯菌门、硝化螺旋菌门和酸杆菌门的相对丰度增加,变形菌门和拟杆菌门的相对丰度减少;而其根系土壤硝化螺旋菌门、拟杆菌门和变形菌门的相对丰度增加,绿弯菌门和酸杆菌门的相对丰度减少。主要优势菌属为酸杆菌属、亚硝化单胞菌属、硝化螺旋菌属、地杆菌属、芽单胞菌属、黄单胞菌属6类。其中亚硝化单胞菌属、硝化螺旋菌属、芽单胞菌属和酸杆菌属在两种模式的表层土壤中的相对丰度存在显著差异;硝化螺旋菌属和地杆菌属在根系土壤中的相对丰度存在显著差异。引入养殖鳖(R-T)后,稻田根系和表层土壤的细菌Chao指数及Shannon指数均增加。PCA以及聚类分析结果显示,养殖鳖引入后,稻田表层土壤的细菌群落结构产生明显变化,同时也在一定程度上对水稻根系的菌群产生了影响。研究初步揭示了稻鳖共作复合生态系统优于稻田单作系统的微生物生态基础。
|
| 关 键 词： | 稻鳖共作 稻田土壤 群落结构 16S rDNA 高通量测序 |
| 收稿时间： | 2017/3/2 0:00:00 |
| 修稿时间： | 2017/4/23 0:00:00 |
Effects of the introduction of cultured turtle on soil bacterial community structure in paddy field |
| |
| LUO Heng,ZHAO Liangjie,LI Feng,GUO Haisong,SHEN Hong and LIU Qigen.Effects of the introduction of cultured turtle on soil bacterial community structure in paddy field[J].Journal of Fisheries of China,2018,42(5):720-732. |
| |
| Authors: | LUO Heng ZHAO Liangjie LI Feng GUO Haisong SHEN Hong and LIU Qigen |
| |
| Institution: | Key Laboratory of Aquatic Genetic Resource and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China,Fishery Biological Engineering Technology Research Center, Henan Province, Xinyang 464000, China,Key Laboratory of Aquatic Genetic Resource and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Aquatic Genetic Resource and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China,Shanghai Mooyuu Farm, Shanghai 202150, China and Key Laboratory of Aquatic Genetic Resource and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China |
| |
| Abstract: | The integrated agri-aquaculture system (IAAS) has become one of the main directions for the sustainable development of agriculture because of its good ecological environmental effect and the ability to produce enough green rice to meet the market demand for high quality food. It is important to understand the structural characteristics of soil bacterial community in this compound system for the improvement of planting and breeding and the realization of ecological regulation. In this study, Illumina high-throughput sequencing was used to compare the surface soil and rhizosphere soil bacterial community structure among rice-turtle co-culture (R-T) and rice monoculture (R-M), and provide the theoretical basis for the accumulation of data of rice-turtle co-culture ecology and the optimization of production technology for comprehensive cultivation of rice turtle co-culture. The results showed that Proteobacteria, Acidobacteria, Bacteroidetes, Nitrospirae and Chloroflexi were the main phyla in all paddy soil. Compared with R-M, the relative abundance of Chloroflexi, Nitrospirae and Acidobacteria increased and the relative abundance of Proteobacteria and Bacteroidetes was reduced in the surface soil of the R-T. In rhizosphere soil, compared with R-M, the relative abundance of Nitrospirae, Bacteroidetes and Proteobacter increased, and the relative abundance of Chloroflexi and Acidobacteria reduced in R-T treatments. The main genera were Acidobacteria bacterium_uncultured, Nitrosomonadaceae_uncultured, Nitrospiraceae_uncultured, Geobacter, Gemmatimonadaceae_uncultured and Xanthomonadales_norank. In the two models, the relative abundance of Nitrospiraceae_uncultured and Geobacter in rhizosphere soil was significantly different (P<0.05), and the relative abundance of Nitrosomonadaceae_uncultured, Nitrospiraceae_uncultured, Gemmatimonadaceae_uncultured and Acidobacteria bacterium_uncultured in the surface soils was significantly different (P<0.05). PCA and cluster analysis showed that R-T treatments exerted a significant effect on the bacterial community structure of surface soils, and also affected the rhizosphere soil bacterial community structure to a certain extent. Overall, this study provides microbial ecological evidences for the advantages of rice - turtle integrated system over rice monoculture. |
| |
| Keywords: | rice-turtle co-culture paddy field community structure 16S rDNA illumina high-throughput sequencing |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
