| 6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响 |
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| 引用本文： | 袁玉辉,杨柳,刘显军,陆赢,刘忠松.6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响[J].作物研究,2013,27(2):121-124. |
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| 作者姓名： | 袁玉辉 杨柳 刘显军 陆赢 刘忠松 |
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| 作者单位： | 湖南农业大学油料作物研究所/国家油料作物改良中心,长沙,410128 |
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| 摘 要： | 以芥菜型油菜品种Brown mustard的下胚轴为外植体,接种于不同配比6-苄基腺嘌呤（6-BA）和硝酸银（AgNO3）组合的分化培养基中,研究6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响。结果表明分化培养基中含3 mg/L 6-BA＋5 mg/L AgNO3时出芽率最高,出芽率达到58.33%,芽丛数平均为1.73。
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| 关 键 词： | 芥菜型油菜 下胚轴 芽再生 6-BA AgNO3 |
Effects of 6-BA and AgNO3 on Shoot Regeneration of Brassica juncea(L.) Hypocotyls |
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| YUAN Yu-hui
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YANG Liu
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LIU Xian-jun
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Lu Ying
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LIU Zhong-song.Effects of 6-BA and AgNO3 on Shoot Regeneration of Brassica juncea(L.) Hypocotyls[J].Crop Research,2013,27(2):121-124. |
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| Authors: | YUAN Yu-hui YANG Liu LIU Xian-jun Lu Ying LIU Zhong-song |
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| Institution: | YUAN Yu-hui, YANG Liu, LIU Xian-jun, Lu Ying, LIU Zhong--song(Oilseed Crops Institute/National Oil Crops Improvement Center, Hunan Agricultural University ,Changsha, Hunan 410128 ,China) |
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| Abstract: | In order to study the effects of 6 -BA and AgNO3 on shoot regeneration of Brassica juncea（ L. ） hypocotyls, we used the Brown mustard （Brassica juncea L. ） hypocotyls as explant, which inoculated in differentiation medium of different concentration ratios of 6 - benzyl adenine （ 6 - BA） and silver nitrate （ AgNO3 ） combination. The results revealed that the shoot regeneration rate was the highest when differentiation medium contained 3 mg/L 6 - BA and 5 mg/L AgNO3, the bud rate reached 58. 33% and the average bud number was 1.73. |
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| Keywords: | Brassica juncea L Hypocotyls Shoot regeneration 6-BA AgNO3 |
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