| 饲料中T-2毒素直接竞争ELISA检测方法的建立 |
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| 引用本文： | 冯才伟,郝士元,孙满义,马腊腊,刘琳,王建霞.饲料中T-2毒素直接竞争ELISA检测方法的建立[J].河南农业科学,2013,42(1):110-113. |
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| 作者姓名： | 冯才伟 郝士元 孙满义 马腊腊 刘琳 王建霞 |
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| 作者单位： | 1. 北京勤邦生物技术有限公司,北京,102206
2. 安佑(中国)动物营养研发有限公司,江苏太仓,215437 |
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| 摘 要： | 采用直接竞争ELISA方法建立了一种快速检测饲料样本中T-2毒素残留的方法,在0～81μg/L范围内,T-2毒素的Logit(B/B0)与T-2毒素质量浓度的对数呈显著的线性关系,并对该检测体系的检测限、准确度、精密度等性能进行了测定。结果显示,该方法的IC50(半数抑制质量浓度)为2.8μg/L,对饲料样本的检测限为10μg/kg,样本添加回收率为77.6%～98.2%,变异系数为5.1%～12.0%。该方法操作简便、准确,检测时间短(仅需15min),适用于饲料样本中T-2毒素的快速检测。
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| 关 键 词： | 直接竞争酶联免疫法 T-2毒素 饲料 检测 |
Establishment of Direct Competitive ELISA for Detecting T-2 Toxin in Animal Feeds |
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| FENG Cai-wei
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HAO Shi-yuan
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SUN Man-yi
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MA La-la
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LIU Lin
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WANG Jian-xia.Establishment of Direct Competitive ELISA for Detecting T-2 Toxin in Animal Feeds[J].Journal of Henan Agricultural Sciences,2013,42(1):110-113. |
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| Authors: | FENG Cai-wei HAO Shi-yuan SUN Man-yi MA La-la LIU Lin WANG Jian-xia |
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| Institution: | 1(1.Beijing Kwinbon Biotechnology Co.,Ltd.,Beijing 102206,China; 2.Anyou(China) Research of Animal Nutrition Co.,Ltd.,Taicang 215437,China) |
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| Abstract: | For detecting the content of T-2 toxin in animal feeds,a direct competitive enzyme-linked immunosorbent assay(ELISA) was established in this study.The results showed that in the concentrate range of 0-81 μg/L,Logit(B/B0) of T-2 toxin showed a significant liner relationship with the logarithm value of T-2 toxin.The IC50 of the established method was 2.8 μg/L and the detection limit of feed was 10μg/kg.The sample recoveries and the coefficients of variation were 77.6%-98.2% and 5.1%-12.0%,respectively.The data indicates that this ELISA kit is convenient,accurate,and can be used for the rapid screening analysis of T-2 toxin in animal feeds. |
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| Keywords: | direct competitive ELISA T-2 toxin feed detection |
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