A Comparison of Microbial Community Function and Structure in Rehabilitated Asbestos and Coal Discard Sites |
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| Authors: | Sarina Claassens Peet Jansen van Rensburg Danica Liebenberg Leon van Rensburg |
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| Institution: | (1) School of Environmental Sciences and Development, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom, 2520, South Africa;(2) School of Physical and Chemical Sciences, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom, 2520, South Africa |
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| Abstract: | Previous studies have made some progress with the use of microbial community properties as assessment criteria for rehabilitation
success of post-mining areas. Currently, there is a need for reference ranges of specific properties in rehabilitated post-mining
sites to make this approach more practical. The aim of this investigation was to compare assessment parameters indicative
of microbial community function (enzymatic assays) and structure (phospholipid fatty acid (PLFA) analysis) in rehabilitated
asbestos and coal discard sites and to establish ranges of minimum and maximum values for these parameters in both types of
sites. The range established for dehydrogenase activity in coal discard sites was 24.3–339.5 μg INF g−1 2 h−1 and for asbestos 44.5–544.6 μg INF g−1 2 h−1. Ranges were also established for β-glucosidase, urease, acid phosphatase and alkaline phosphatase. Complete PLFA profiles
were determined and ranges established for major PLFA groups and ratios in both types of discard. From the PLFA profiles,
viable microbial biomass was determined as 6,080–29,851 and 8,128–47,242 pmol g−1 dry weight for the coal and asbestos discard sites, respectively. While similar ranges were observed for both types of discard,
a canonical correspondence analysis that accounts for functional and structural characteristics showed that sites clustered
according to the origin of the samples. |
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