Application of DNA markers in parentage verification of Boran cattle in Kenya |
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| Authors: | David Kios Estè van Marle-Köster Carina Visser |
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| Institution: | (1) Department of Animal and Wildlife Sciences, University of Pretoria, Pretoria, 0002, Republic of South Africa;(2) Chepkoilel University College, Department of Animal Science, Moi University, P.O. Box 1125, 30100 Eldoret, Kenya |
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| Abstract: | Boran cattle provide livelihood to thousands of households in the arid and semiarid lands of Kenya. Due to their superior
adaptive and productive traits in comparison to other breeds of cattle, they have also become a popular choice for breeders
in Eastern and Southern Africa. Continued genetic improvement of the breed is important, and therefore accurate performance
and pedigree records are required. One hundred seventy-eight pedigree records and blood samples of four Boran stud herds were
evaluated for accuracy of parentage allocation using 11 microsatellite markers recommended by ISAG for parentage verification.
The panel of the 11 microsatellite markers was found to be highly polymorphic (PIC of 0.6901) with a combined probability
of exclusion of 0.9997. The dam misidentification was low ranging between 0% and 5% for the herds tested. The estimated rate
of mispaternity however ranged between 4.3% and 80% among the four stud herds, and more than 50% of the offspring of some
herds were misidentified. The high rate of mispaternity will have a negative impact on the response to selection. The use
of DNA markers for parentage assignment will improve the accuracy of the pedigree records of Boran stud cattle in Kenya and
contribute to more accurate selection of superior animals. |
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