| 液相色谱串联质谱法测定茶叶中氨基甲酸酯类物质研究 |
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| 引用本文： | 顾亮,丁磊.液相色谱串联质谱法测定茶叶中氨基甲酸酯类物质研究[J].现代农业科技,2012(15):286-287,291. |
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| 作者姓名： | 顾亮 丁磊 |
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| 作者单位： | 安徽国家农业标准化与监测中心,安徽合肥,230051 |
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| 摘 要： | 对液相色谱-串联质谱法(LC-ESI-MS/MS)快速准确测定茶叶中灭多威、克百威、涕灭威、甲萘威含量进行研究。对样品前处理方法进行了优化,样品经乙腈提取、氨基固相萃取柱净化后,采用HPLC-ESI-MS/MS检测分析,在多反应监测模式(MRM)下,外标法定量。结果表明:方法的检出限为5μg/kg,样品浓度为0.001～0.200 mg/kg时线性关系良好(相关系数r0.99),平均添加回收率70%～75%。该方法简便、高效、准确,各项技术指标均满足国内外有关茶叶中农药残留限量要求,可用于茶叶中灭多威、克百威、涕灭威、甲萘威残留的分析测定。
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| 关 键 词： | 高效液相色谱串联质谱 茶叶 氨基甲酸酯类 测定 |
Study on Determination of Carbamate Material in Tea by Liquid Chroma |
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| Authors: | GU Liang DING Lei |
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| Institution: | (The Center of Anhui National Agricultural Standardization and Monitoring,Hefei Anhui 230051) |
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| Abstract: | A new method using of ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry for the analysis of Thiodicarb、Carbofuran、Aldicarb and Carbaryl was studied.Sample preparation and HPLC-MS/MS analytical conditions were examined and optimized,Samples were extracted by acetonitrile,then cleaned up,and ultimately adopted LC-ESI-MS/MS method for qualitative and quantitative analysis(MRM).Results showed that,limits of quantification(LOQ) and limits of detection(LOD) was 5 μg/kg,the method showed good linearity(r>0.99)when sample concentrations were from 0.001 to 0.200 mg/kg.The average recoveries were from 70% to 75%.The method was simple,efficient,sensitive and accurate,which could meet the requirements of determination for Thiodicarb,Carbofuran,Aldicarb and Carbaryl of tea. |
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| Keywords: | liquid chromatography-electrospray ionization tandem mass spectrometry tea carbamate material determination |
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