The inheritance of chemical phenotype in <Emphasis Type="Italic">Cannabis</Emphasis><Emphasis Type="Italic">sativa</Emphasis> L. (IV): cannabinoid-free plants |
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| Authors: | E P M de Meijer K M Hammond A Sutton |
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| Institution: | (1) GW Pharmaceuticals Plc., Porton Down Science Park, Salisbury, Wiltshire, SP4 0JQ, UK |
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| Abstract: | A genetic factor that blocks the cannabinoid biosynthesis in Cannabis
sativa has been investigated. Crosses between cannabinoid-free material and high content, pharmaceutical clones were performed.
F1s were uniform and had cannabinoid contents much lower than the mean parental value. Inbred F2 progenies segregated into discrete groups: a cannabinoid-free chemotype, a chemotype with relatively low cannabinoid content
and one with relatively high content, in a monogenic 1:2:1 ratio. In our model the cannabinoid knockout factor is indicated
as a recessive allele o, situated at locus O, which segregates independently from previously presented chemotype loci. The genotype o/o underlies the cannabinoid-free chemotype, O/o is expressed as an intermediate, low content chemotype, and O/O is the genotype of the high content chemotype. The data suggests that locus O governs a reaction in the pathway towards the phenolic cannabinoid precursors. The composition of terpenoids and various
other compound classes of cannabinoid-free segregants remains unaffected. Backcrossing produced cannabinoid-free homologues
of pharmaceutical production clones with potential applications in pharmacological research. A new variant of the previously
presented allele ‘B
0’, that almost completely obstructs the conversion of CBG into CBD, was also selected from the source population of the cannabinoid
knockout factor. |
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| Keywords: | Cannabinoids Cannabis Chemotype Genotype Knockouts |
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