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Optimal chemical triploid induction in geoduck clams, Panopea abrupta, by 6-dimethylaminopurine |
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| Authors: | Brent Vadopalas Jonathan P Davis |
| Institution: | a Marine Molecular Biotechnology Laboratory, School of Aquatic and Fishery Sciences, University of Washington, 1222 Boat St., Seattle, WA 98115, USA b Baywater Inc., 15425 Smoland Lane, Bainbridge Island, WA 98110, USA |
| Abstract: | The development of geoduck culture techniques coupled with increased market demand during the past decade led to cultured intertidal geoduck beds, over which concerns have arisen regarding the potential genetic risk posed by the reproductive contribution of hatchery outplants to wild stocks. Although an ongoing study to determine the genetic stock structure of Puget Sound geoducks has yet to yield definitive results, sterile triploids would enable geoduck culture to proceed irrespective of any genetic differences found, and may increase rate of growth. At 15 °C and salinity of 30‰, we evaluated the suitability of cytochalasin B (CB) and 6-dimethylaminopurine (6-DMAP) for triploid induction in geoducks. Parallel tests of induction agent, start time, duration, and concentration were conducted on the same gamete pool. We found optimal triploid induction (92%) and suitable survivorship (30%) resulted from a 600 μM 6-DMAP treatment. This work enables the future examination of the efficacy of triploid induction for conferring sterility and increasing growth rate in this commercially valuable species. |
| Keywords: | Panopea abrupta Geoduck Triploid 6-DMAP CB |
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