| 通过分子标记辅助选择培育优良食味水稻新品种南粳46(英文) |
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| 引用本文： | 王才林,张亚东,朱镇,陈涛,赵凌,林静,周丽慧.通过分子标记辅助选择培育优良食味水稻新品种南粳46(英文)[J].分子植物育种,2009,7(6):1070-1076. |
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| 作者姓名： | 王才林 张亚东 朱镇 陈涛 赵凌 林静 周丽慧 |
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| 作者单位： | 江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,南京,210014 |
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| 基金项目： | This research was supported by the Key Program of the Development of Variety of Genetically Modified Organisms，the Key Support Program of Jiangsu Science and Technology，Jiangsu Agricultural Scientific Self-innovation Fund |
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| 摘 要： | 为培育食味品质优良、综合丰产性好的粳稻新品种,本研究以高产粳稻品种武香粳14作母本,以食味品质优良粳稻品种关东194(含有控制低直链淀粉含量和暗胚乳的突变基因Wx-mq)作父本配制杂交组合.通过比较Wx-mq与其位于第6染色体上的等位基因Wx-b,Wx-a,wx的DNA序列,发现Wx-mq基因的外显子4和外显子5上存在两个碱基突变,外显子4上的G-A突变产生了NIaⅢ的酶切位点,并设计合成了能区分含或不含Wx-mq纯合基因型和杂合基因型的CAPS标记.利用该标记对武香粳14/关东194衍生的F_5、F_6株系进行辅助选择,筛选含Wx-mq纯合基因型的单株.成熟后对胚乳淀粉性质的调查结果与分子检测结果完全一致,分子标记辅助选择效果达100%.这样我们就成功培育了食味品质优良、综合丰产性好的粳稻新品种南粳46.
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| 关 键 词： | 分子标记辅助选择 优良食味 水稻 酶切扩增多态性序列 |
Development of a New Japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection |
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| Wang Cailin,Zhang Yadong,Zhu Zhen,Chen Tao,Zhao Ling,Lin Jing,Zhou Lihui.Development of a New Japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection[J].Molecular Plant Breeding,2009,7(6):1070-1076. |
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| Authors: | Wang Cailin Zhang Yadong Zhu Zhen Chen Tao Zhao Ling Lin Jing Zhou Lihui |
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| Institution: | Wang Cailin Zhang Yadong Zhu Zhen Chen Tao Zhao Ling Lin Jing Zhou Lihui Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center,Nanjing Branch of China National Center for Rice Improvement,Nanjing,210014 |
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| Abstract: | In order to developing a new japonica rice variety with both good eating quality and high yield,we employed the high yield japonica rice Wu-xiang-jing 14as the maternal parent was crossed to the good quality japonica riceKantou 194with low-amylose content and translucent endosperm controlled by Wx-mq.By comparing the DNA sequence of Wx-mq gene and its allelic genes Wx-b,Wx-a,wx in chromosome 6,two mutated bases occurring in exon 4 and exon 5 were found in Wx-mq.The change of G-A in exon 4 just produced a re... |
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| Keywords: | Marker assisted selection Good eating quality Rice Cleaved amplified polymorphic sequences(CAPS) |
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