| 姬菇杂交菌株的ISSR分析 |
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| 引用本文： | 周洁,郭勇,贾定洪,谭伟,张小平,郑林用,彭卫红,甘炳成,黄忠乾.姬菇杂交菌株的ISSR分析[J].西南农业学报,2009,22(6). |
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| 作者姓名： | 周洁 郭勇 贾定洪 谭伟 张小平 郑林用 彭卫红 甘炳成 黄忠乾 |
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| 作者单位： | 1. 四川农业大学资源环境学院,四川,雅安,625014
2. 四川省农业科学院土壤肥料研究所,四川,成都,610066 |
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| 基金项目： | 四川省农作物育种攻关计划子专题，四川省学术和技术带头人培养资金项目，国家农业产业技术体系四川食用菌创新团队新品种引育研究岗位建设项目 |
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| 摘 要： | 采用ISSR标记技术对40个姬菇杂交菌株及2个亲本菌株进行分析,使用NTSYSpc2.1生物软件对42个供试菌株进行聚类分析,构建系统树.试验筛选出了11个扩增谱带清晰、多态性好的ISSR引物,共扩增出81条清晰易辨的多态性谱带.聚类分析结果表明,42个供试菌株遗传相似系数变异范围为0.4286～0.8537,在相似系数0.6680时,42个菌株聚为7类,两亲本各聚一类,杂交菌株与亲本菌株遗传差异较大.
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| 关 键 词： | 姬菇 杂交菌株 亲本 |
Hybrid Strains of Pleurotus cornucopiae Identified by ISSR |
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| ZHOU Jie,GUO Yong,JIA Ding-hong,TAN Wei,ZHANG Xiao-ping,ZHENG Lin-yong,PENG Wei-hong,GAN Bing-cheng,HUANG Zhong-qian.Hybrid Strains of Pleurotus cornucopiae Identified by ISSR[J].Southwest China Journal of Agricultural Sciences,2009,22(6). |
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| Authors: | ZHOU Jie GUO Yong JIA Ding-hong TAN Wei ZHANG Xiao-ping ZHENG Lin-yong PENG Wei-hong GAN Bing-cheng HUANG Zhong-qian |
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| Abstract: | Inter-simple sequence repeat (ISSR) was used to determine the forty hybrid strains and their parents strains (J1-2, J2-1).Genetic similarity coefficient and the dendrogram were obtained by NTSYS-pc(Version 2.10e)software. 11 primers which were screened from 75 ISSR primers could be used to amplify the genomes of all these 42 test strains, and to gain the clear and stable fingerprint maps with strong polymorphism. The result showed that the total 116 bands were gained, and the similarity coefficients ranged from 0.4286 to 0.8537. When the similarity coefficient was 0.6680, forty-two strains separated in 7 glades were different from their diverse parents. |
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| Keywords: | ISSR P cornucopiae Hybrid strains Parents strains ISSR |
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