| 大豆LAZ1基因家族鉴定与GmLAZ1-9基因的功能研究 |
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| 引用本文： | 杨昕霞,张斌.大豆LAZ1基因家族鉴定与GmLAZ1-9基因的功能研究[J].浙江农业学报,2021,33(4):586. |
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| 作者姓名： | 杨昕霞 张斌 |
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| 作者单位： | a. 后勤处 湖南科技学院b.化学与生物工程学院,湖南 永州 425199 |
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| 基金项目： | 湖南省自然科学基金(2020JJ4030) |
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| 摘 要： | 通过在大豆基因组数据库中比对,获得大豆LAZ1基因家族成员,开展生物信息学分析与表达模式检测,并利用转基因拟南芥进行基因功能验证。结果显示:共鉴定到17个大豆LAZ1基因,即GmLAZ1-1~ GmLAZ1-17。这17个GmLAZ1基因不均匀地分布在大豆的12条染色体上,在进化树上可分为3个亚家族,同一亚家族成员具有类似的基因和蛋白结构。顺式作用元件预测结果表明,大豆LAZ1家族基因启动子上存在很多与非生物胁迫相关的元件,如ABRE、ARE、LTR、G-box等。半定量PCR检测结果显示,GmLAZ1-5、GmLAZ1-9、GmLAZ1-11和GmLAZ1-13在盐处理后的大豆幼苗中表达上调。在拟南芥中过表达GmLAZ1-9基因增强了转基因植株的耐盐性。这些发现可为进一步探究其他LAZ1基因的功能与分子机制提供依据。
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| 关 键 词： | 大豆 LAZ1家族 盐胁迫 基因表达 |
| 收稿时间： | 2020-10-11 |
Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9 |
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| YANG Xinxia,ZHANG Bin.Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9[J].Acta Agriculturae Zhejiangensis,2021,33(4):586. |
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| Authors: | YANG Xinxia ZHANG Bin |
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| Institution: | a. Department of Logistics
b. College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China |
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| Abstract: | In the present study, the BLAST search was performed in the soybean genome database and LAZ1 gene family members were obtained. Bioinformatics analysis and expression pattern detection were performed, and transgenic Arabidopsis was used to verify gene function. It was shown that a total of 17 soybean LAZ1 genes were identified, namely GmLAZ1-1 to GmLAZ1-17. All of the GmLAZ1 genes were unevenly distributed on 12 chromosomes. The LAZ1 family members in soybean were phylogenetically divided into three evolutionary clades, and the members in the same clades shared similar gene and protein structures. The analysis of cis-regulatory elements indicated that multiple abiotic stress-related cis-acting elements presented in the promoters of soybean LAZ1 genes, such as ABRE, ARE, LTR, G-box, etc. Semi-quantitative PCR results showed that the expression of GmLAZ1-5, GmLAZ1-9, GmLAZ1-11, and GmLAZ1-13 was up-regulated after salt treatment. Moreover, GmLAZ1-9 enhanced salinity tolerance in transgenic Arabidopsis, which provided basis for further exploration of the functions and molecular mechanisms of other LAZ1 genes. |
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| Keywords: | soybean LAZ1 family salt stress gene expression |
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