| 贵州核桃农家品种种质资源遗传多样性的SRAP分析 |
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| 引用本文： | 杨小红,侯娜.贵州核桃农家品种种质资源遗传多样性的SRAP分析[J].种子,2021(1):46-49. |
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| 作者姓名： | 杨小红 侯娜 |
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| 作者单位： | 贵州省林业科学研究院;贵州省核桃研究所 |
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| 基金项目： | 国家自然科学基金项目(31860215);中央财政推广示范项目([2018]TG 03);贵州省科技厅基金项目(黔科合J字[2014]2105号);贵州省科技厅农业领域“工程技术研究中心”项目(黔科合平台人才[2019]5202号);贵州省科技厅基金项目(黔科合基础[2018]1092号);贵州省科技厅农业攻关计划(黔科合支撑[2016]2572号);贵州省科技厅科技计划(黔科合支撑[2019]2330号);贵州省林业局科技计划(黔林科合J字[2019]12号);贵州省科技计划(黔科合成果[2018]4320号)。 |
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| 摘 要： | 采用SRAP分子标记技术对采自贵州省盘州市、普安县、贵阳市、兴义市、毕节市、威宁县、沿河县种源地的168份核桃农家种样品进行遗传多样性分析.结果表明,从132对引物组合中筛选出了19对图谱带型清晰、丰富、重复性好的引物组合;168份样品用这些引物组合共扩增出309条清晰可重复的条带,其中多态性条带281条,多态性比率为...
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| 关 键 词： | 贵州核桃 SRAP分子标记 遗传多样性 |
Genetic Diversity Analysis of Native Walnut Germplasms in Guizhou by SRAP Marker |
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| YANG Xiaohong,HOU Na.Genetic Diversity Analysis of Native Walnut Germplasms in Guizhou by SRAP Marker[J].Seed,2021(1):46-49. |
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| Authors: | YANG Xiaohong HOU Na |
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| Institution: | (Guizhou Academy of Forestry,Guiyang 550005,China;Guizhou Institute of Walnut,Guiyang 550005,China) |
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| Abstract: | Genetic diversity of the 168 native walnut germplasms collected from Panxian,Pu’an,Guiyang,Xingyi,Bijie,Weining and Yanhe in Guizhou was analyzed using SRAP marker.The results showed that 19 pairs of primer screened out from 132 pairs of primer combinations could amplify clear,rich,and good repetitive bands,and a total of 309 clear and repeatable bands were amplified from 168 samples using these primer combinations.Among them,281 bands were polymorphic,and the polymorphism ratio was 90.94%.The results of cluster analysis showed that at the similarity coefficient of 0.62,all walnut germplasms were clustered into two groups,while at the similarity coefficient of 0.72,these walnut germplasms were clustered into ten groups,which indicated that these native walnut germplasms were related to each other.Additionally,the walnut germplasm from Xingyi had the highest level of genetic diversity among the germplasms studied. |
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| Keywords: | guizhou walnuts SRAP marker genetic diversity |
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