| 除草活性菌株HL-1产孢发酵条件研究 |
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| 引用本文： | 高汉峰,刘雨芹,程亮,郭青云.除草活性菌株HL-1产孢发酵条件研究[J].浙江农业学报,2021,33(6):1042. |
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| 作者姓名： | 高汉峰 刘雨芹 程亮 郭青云 |
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| 作者单位： | 青海大学农林科学院 植物保护研究所,青海省农业有害生物综合治理重点实验室,农业农村部西宁作物有害生物科学观测实验站,青海 西宁 810016 |
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| 基金项目： | 国家自然科学基金(31760539);青海省科技厅项目(2018-ZJ-917);第二次青藏高原综合科学考察研究项目(2019QZKK0303) |
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| 摘 要： | 以分离自青海患病刺儿菜中具有除草活性的植物内生真菌HL-1菌株为研究对象,通过单因素试验筛选最适合该菌株生长的碳氮源、固态发酵基质、接种量和固-液发酵条件。结果表明:在供试的材料中最适碳源是葡萄糖,氮源是酵母浸膏;该菌株在PDA培养基中的最大产孢量是5.79×108 CFU·mL-1;而在最适碳源和最适氮源混合培养基中,它的最大产孢量是 4.81×109 CFU·mL-1。菜籽饼为HL-1在供试材料中产孢最大的培养基质;在体积分数为40%接种量下,它的产孢量最大。通过正交优化试验发现该菌株在培养温度27.5 ℃,培养基质含水量37%,发酵时间8 d条件下,产孢量最大。
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| 关 键 词： | 植物内生真菌HL-1 固态发酵基质 固-液发酵条件 |
| 收稿时间： | 2020-06-29 |
Study on fermentation conditions of herbicidal active strain HL-1 |
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| GAO Hanfeng,LIU Yuqin,CHENG Liang,GUO Qingyun.Study on fermentation conditions of herbicidal active strain HL-1[J].Acta Agriculturae Zhejiangensis,2021,33(6):1042. |
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| Authors: | GAO Hanfeng LIU Yuqin CHENG Liang GUO Qingyun |
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| Institution: | Institute of Plant Protection, Academy of Agriculture and Forestry Science, Qinghai University, Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Scientific Observing and Experimental Station of Crop Pest of Ministry of Agriculture and Rural Affairs in Xining, Xining 810016, China |
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| Abstract: | Taking endophytic fungus HL-1 strain with herbicidal activity isolated from Qinghai diseased Cirsium setosum as the material, a single factor test was used to screen the carbon and nitrogen sources, solid fermentation substrate, inoculation amount and solid-liquid fermentation conditions that were most suitable for the growth of the strain. The results showed that the most suitable carbon source of HL-1 in the tested materials was glucose and the nitrogen source was yeast extract; when the strain was cultured in PDA medium, its maximum spore production was 5.79×108 CFU·mL-1 ; and in the optimal carbon source and optimal nitrogen source mixed medium, its maximum spore production was 4.81×109 CFU·mL-1. Rapeseed cake was the medium material with the largest spore production of HL-1 in the test materials, which had the largest spore production at a volume fraction of 40% inoculation volume. Through orthogonal optimization experiments, the spore production of the strain was found to be the largest under the conditions of culture temperature 27.5 ℃, culture medium water content 37% and fermentation time 8 d. |
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| Keywords: | endophytic fungus HL-1 solid fermentation substrate solid-liquid fermentation conditions |
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